Document Detail


Role of uremic and endothelial factors in the development of beta 2-microglobulin amyloidosis.
MedLine Citation:
PMID:  8320954     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To clarify the importance of uremic and endothelial factors in the development of beta 2-microglobulin (beta 2m) amyloidosis, in vitro evaluations were carried out using cultured synoviocytes and synovial tissues. Synoviocytes of patients with chronic renal failure were cultured with media containing uremic or normal serum. In cultures with uremic serum, greater proliferation of synoviocytes and stronger expression of CD68, beta 2m, interleukin 1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF alpha) on synoviocytes were observed, compared with those containing normal serum. Uremic serum stimulated greater production of IL-1 beta and TNF alpha than normal serum (P < 0.05 for IL-1 beta, P < 0.01 for TNF alpha). Addition of supernatant of endothelial cell culture to medium containing uremic serum significantly accelerated production. After a three month culture of uremic synovial tissue, embedded in 3% type 1 collagen gel with uremic serum, beta 2m-positive 10 nm fibrils were recognized around the synoviocytes. In culture with uremic serum plus supernatant of endothelial cell culture, positive Congo red staining was noted. These findings indicate that uremic serum plays a significant role in the development of synovial beta 2m amyloidosis and that endothelial cell-derived factors contribute to the formation of beta 2m amyloid.
Authors:
Y Nakashima; T Akizawa; T Nagai; S Koshikawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Kidney international. Supplement     Volume:  41     ISSN:  0098-6577     ISO Abbreviation:  Kidney Int. Suppl.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-08-05     Completed Date:  1993-08-05     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7508622     Medline TA:  Kidney Int Suppl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  S88-95     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Showa University, Fujigaoka Hospital, Yokohama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amyloidosis / etiology*
Cell Division
Cells, Cultured
Endothelium, Vascular / physiology*
Humans
Interleukin-1 / biosynthesis
Synovial Membrane / cytology,  metabolism
Tumor Necrosis Factor-alpha / biosynthesis
Uremia / complications*
beta 2-Microglobulin / metabolism*
Chemical
Reg. No./Substance:
0/Interleukin-1; 0/Tumor Necrosis Factor-alpha; 0/beta 2-Microglobulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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