| Role of tumour necrosis factor-{alpha} (TNF{alpha}) in the functional properties of hyalocytes. | |
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MedLine Citation:
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PMID: 21030411 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background/aim Tumour necrosis factor-α (TNFα) is an inflammatory cytokine that is upregulated in various vitreoretinal diseases including uveitis and diabetic retinopathy. Recently, our studies have indicated that hyalocytes contribute to the pathogenesis of these diseases. However, the impact of TNFα on the functional properties of hyalocytes is unknown. Methods Hyalocytes were isolated from bovine eyes. Cellular proliferation, migration and gel contraction in response to TNFα and the other inflammatory cytokines were analysed by thymidine uptake, Boyden's chamber assay and collagen gel contraction assay, respectively. Furthermore, we estimated the effect of dexamethasone on these properties of hyalocytes. Results TNFα promoted proliferation, migration and gel contraction by hyalocytes. Dexamethasone inhibited TNFα-induced proliferation but not migration. Dexamethasone did not inhibit TNFα-induced gel contraction but further increased contraction. Furthermore, dexamethasone inhibited TNFα-induced extracellular signal-related kinase (ERK)1/2 phosphorylation in hyalocytes. Conclusion This study indicates that TNFα in vitreous and retina causes activation of hyalocytes, and the activated hyalocytes contribute to the pathogenesis of inflammatory vitreoretinal diseases. Steroid treatment appears to inhibit the activation of hyalocytes in the early stages of the diseases, but might have adverse effects in the late stage through membrane contraction. |
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Authors:
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Yasuaki Hata; Shintaro Nakao; Ri-Ichiro Kohno; Kumiyo Oba; Takeshi Kita; Muneki Miura; Yukio Sassa; Alexander Schering; Tatsuro Ishibashi |
Publication Detail:
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Type: Journal Article Date: 2010-10-28 |
Journal Detail:
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Title: The British journal of ophthalmology Volume: 95 ISSN: 1468-2079 ISO Abbreviation: Br J Ophthalmol Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0421041 Medline TA: Br J Ophthalmol Country: England |
Other Details:
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Languages: eng Pagination: 261-5 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan; ishi@med.kyushu-u.ac.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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