| THE ROLE OF TOLL-LIKE RECEPTOR 4 IN CORNEAL EPITHELIAL WOUND HEALING. | |
MedLine Citation:
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PMID: 25183764 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose: To evaluate the role of TLR4 in corneal epithelial wound healing. Methods: The expression of TLR4 during in vivo corneal epithelial wound healing was examined by immunostaining in mice. The expression and activation of TLR4 was studied in primary or telomerase-immortalized human corneal epithelial cells (HCEC). Scratch assay was performed to evaluate in vitro wound closure using live time-lapse microscopy. Transwell migration assay and Ki67 immunostaining were done to evaluate migration and proliferation, respectively. Lipopolysaccharide (LPS) was used to activate TLR4 whereas CLI-095 was used for its inhibition. The expression of inflammatory cytokines was determined by RT-PCR and ELISA. The activation of p42/44 and p38 was determined by immunoblotting. Results: In the murine model, TLR4 immunostaining was prominently noted in the epithelium 8 hours after wounding. There was a 4-fold increase in the expression of TLR4 6 hours after in vitro scratch wounding (P<0.001). Confocal microscopy confirmed the membrane localization of TLR4/MD2 complex. There was a significant increase in migration, proliferation and wound closure in HCEC treated with LPS (P<0.05) while there was significant decrease with TLR4 inhibition (P<0.05). Addition of LPS to wounded HCEC resulted in a significant increase in the expression of IL-6, TNF-α, CXCL8/IL8, and CCL5/RANTES both at the mRNA and protein level. Likewise, LPS increased the activation of p42/44 and p38 in wounded HCEC. Conclusions: These results suggest that epithelial wounding induces the expression of functional TLR4. TLR4 signaling appears to contribute to early corneal epithelial wound repair by enhancing migration and proliferation. |
Authors:
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Medi Eslani; Asadolah Movahedan; Neda Afsharkhamseh; Herve Sroussi; Ali R Djalilian |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2014-9-2 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: - ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2014 Sep |
Date Detail:
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Created Date: 2014-9-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2014 by Association for Research in Vision and Ophthalmology. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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