Document Detail


Role of surface roughness of titanium versus hydroxyapatite on human bone marrow cells response.
MedLine Citation:
PMID:  11927830     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was designed to assess human bone marrow cell response and particularly cell adhesion, proliferation, and differentiation, when cultured in vitro on titanium alloy and hydroxyapatite with different values of surface roughness. A further aim was to compare the cell response on the two materials, currently used in spinal surgery. Cell adhesion was determined after 0.5, 2, 4, and 18 hours of incubation; proliferation after 8, 11, 14, and 16 days of culture; and differentiation was evaluated with the expression of alkaline phosphatase activity after 8 and 16 days of culture. This study showed that bone marrow cells grew faster on titanium alloy than on hydroxyapatite, although fewer cells attached to titanium, compared to those attached to hydroxyapatite. No statistically significant difference was observed as the expression of alkaline phosphatase activity on hydroxyapatite and titanium alloy of the same roughness. Cell adhesion, proliferation, and differentiation are dependent on surface roughness of the biomaterial, and all three increased as the roughness of titanium alloy increased. Conclusively, surface roughness of titanium and hydroxyapatite significantly influences bone marrow cell response, and therefore these biomaterials should be used with rough outer surface, if a significant cell response on them is desired. These advantages of titanium and hydroxyapatite theoretically seem to be of particular importance in the following situations: long fusions, lumbosacral fusion, revision surgery with poor bone bank, neuropathic scoliosis associated with few bone graft reserves, and adult patients with severe osteoporosis.
Authors:
Panagiotis G Korovessis; Despina D Deligianni
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of spinal disorders & techniques     Volume:  15     ISSN:  1536-0652     ISO Abbreviation:  J Spinal Disord Tech     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-04-02     Completed Date:  2002-06-13     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  101140323     Medline TA:  J Spinal Disord Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  175-83; discussion 183     Citation Subset:  IM    
Affiliation:
Orthopaedic Department General Hospital, Agios Andreas, Patras, Greece. korovess@otenet.gr
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MeSH Terms
Descriptor/Qualifier:
Bone Marrow Cells* / metabolism
Cell Adhesion
Cell Culture Techniques
Cell Differentiation
Cell Division
Durapatite*
Humans
Spinal Fusion
Surface Properties
Titanium*
Chemical
Reg. No./Substance:
12743-70-3/titanium alloy (TiAl6V4); 1306-06-5/Durapatite; 7440-32-6/Titanium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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