| Role of sterol type on lateral pressure profiles of lipid membranes affecting membrane protein functionality: Comparison between cholesterol, desmosterol, 7-dehydrocholesterol and ketosterol. | |
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MedLine Citation:
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PMID: 17369050 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lateral pressure profiles have been suggested to play a significant role in many cellular membrane processes by affecting, for example, the activation of membrane proteins through changes in their conformational state. This may be the case if the lateral pressure profile is altered due to changes in molecular composition surrounding the protein. In this work, we elucidate the effect of varying sterol type on the lateral pressure profile, an issue of topical interest due to lipid rafts and their putative role for membrane protein functionality. We find that the lateral pressure profile is altered when cholesterol is replaced by either desmosterol, 7-dehydrocholesterol, or ketosterol. The observed changes in the lateral pressure profile are notable and important since desmosterol and 7-dehydrocholesterol are the immediate precursors of cholesterol along its biosynthetic pathway. The results show that the lateral pressure profile and the resulting elastic behavior of lipid membranes are sensitive to the sterol type, and support a mechanism where changes in protein conformational state are facilitated by changes in the lateral pressure profile. From a structural point of view, the results provide compelling evidence that despite seemingly minor differences, sterols are characterized by structural specificity. |
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Authors:
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O H Samuli Ollila; Tomasz Róg; Mikko Karttunen; Ilpo Vattulainen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-02-01 |
Journal Detail:
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Title: Journal of structural biology Volume: 159 ISSN: 1047-8477 ISO Abbreviation: J. Struct. Biol. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-07-30 Completed Date: 2008-02-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9011206 Medline TA: J Struct Biol Country: United States |
Other Details:
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Languages: eng Pagination: 311-23 Citation Subset: IM |
Affiliation:
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Institute of Physics, Tampere University of Technology, P.O. Box 692, FI-33101 Tampere, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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chemistry* Cholesterol / chemistry* Dehydrocholesterols / chemistry* Desmosterol / chemistry* Elasticity Lipid Bilayers / chemistry Membrane Lipids / chemistry Membrane Proteins / metabolism* Molecular Structure Pressure Sterols / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Dehydrocholesterols; 0/Lipid Bilayers; 0/Membrane Lipids; 0/Membrane Proteins; 0/Sterols; 313-04-2/Desmosterol; 434-16-2/7-dehydrocholesterol; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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