Document Detail

Role of sterol type on lateral pressure profiles of lipid membranes affecting membrane protein functionality: Comparison between cholesterol, desmosterol, 7-dehydrocholesterol and ketosterol.
MedLine Citation:
PMID:  17369050     Owner:  NLM     Status:  MEDLINE    
Lateral pressure profiles have been suggested to play a significant role in many cellular membrane processes by affecting, for example, the activation of membrane proteins through changes in their conformational state. This may be the case if the lateral pressure profile is altered due to changes in molecular composition surrounding the protein. In this work, we elucidate the effect of varying sterol type on the lateral pressure profile, an issue of topical interest due to lipid rafts and their putative role for membrane protein functionality. We find that the lateral pressure profile is altered when cholesterol is replaced by either desmosterol, 7-dehydrocholesterol, or ketosterol. The observed changes in the lateral pressure profile are notable and important since desmosterol and 7-dehydrocholesterol are the immediate precursors of cholesterol along its biosynthetic pathway. The results show that the lateral pressure profile and the resulting elastic behavior of lipid membranes are sensitive to the sterol type, and support a mechanism where changes in protein conformational state are facilitated by changes in the lateral pressure profile. From a structural point of view, the results provide compelling evidence that despite seemingly minor differences, sterols are characterized by structural specificity.
O H Samuli Ollila; Tomasz Róg; Mikko Karttunen; Ilpo Vattulainen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-01
Journal Detail:
Title:  Journal of structural biology     Volume:  159     ISSN:  1047-8477     ISO Abbreviation:  J. Struct. Biol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-30     Completed Date:  2008-02-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9011206     Medline TA:  J Struct Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  311-23     Citation Subset:  IM    
Institute of Physics, Tampere University of Technology, P.O. Box 692, FI-33101 Tampere, Finland.
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MeSH Terms
Cell Membrane / chemistry*
Cholesterol / chemistry*
Dehydrocholesterols / chemistry*
Desmosterol / chemistry*
Lipid Bilayers / chemistry
Membrane Lipids / chemistry
Membrane Proteins / metabolism*
Molecular Structure
Sterols / chemistry*
Reg. No./Substance:
0/Dehydrocholesterols; 0/Lipid Bilayers; 0/Membrane Lipids; 0/Membrane Proteins; 0/Sterols; 313-04-2/Desmosterol; 434-16-2/7-dehydrocholesterol; 57-88-5/Cholesterol

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