Document Detail

Role of sphingomyelin and ceramide in the regulation of the activity and fatty acid specificity of group V secretory phospholipase A2.
MedLine Citation:
PMID:  17178097     Owner:  NLM     Status:  MEDLINE    
We previously showed that group V secretory phospholipase A(2) (sPLA(2)V) is inhibited by sphingomyelin (SM), but activated by ceramide. Here, we investigated the effect of sphingolipid structure on the activity and acyl specificity of sPLA(2)V. Degradation of HDL SM to ceramide, but not to ceramide phosphate, stimulated the activity by 6-fold, with the release of all unsaturated fatty acids being affected equally. Ceramide-enrichment of HDL similarly stimulated the release of unsaturated fatty acids. Incorporation of SM into phosphatidylcholine (PC) liposomes preferentially inhibited the hydrolysis of 16:0-20:4 PC. Conversely, SMase C treatment or ceramide incorporation resulted in preferential stimulation of hydrolysis of 16:0-20:4 PC. The presence of a long chain acyl group in ceramide was essential for the activation, and long chain diacylglycerols were also effective. However, ceramide phosphate was inhibitory. These studies show that SM and ceramide in the membranes and lipoproteins not only regulate the activity of phospholipases, but also the release of arachidonate, the precursor of eicosanoids.
Dev K Singh; Laurence R Gesquiere; Papasani V Subbaiah
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-11-21
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  459     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-14     Completed Date:  2007-05-18     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  280-7     Citation Subset:  IM    
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MeSH Terms
Ceramides / chemistry*
Enzyme Activation
Enzyme Stability
Fatty Acids / chemistry*
Group V Phospholipases A2
Phospholipases A / chemistry*
Phospholipases A2
Sphingomyelins / chemistry*
Grant Support
Reg. No./Substance:
0/Ceramides; 0/Fatty Acids; 0/Sphingomyelins; EC 3.1.1.-/Phospholipases A; EC V Phospholipases A2; EC A2

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