Document Detail

Role of skeletal muscle-fibre type in regulation of glucose metabolism in middle-aged subjects with impaired glucose tolerance during a long-term exercise and dietary intervention.
MedLine Citation:
PMID:  16219019     Owner:  NLM     Status:  MEDLINE    
AIM: The aim of this study was to investigate the role of skeletal muscle fibre type in the regulation of glucose metabolism in middle-aged obese subjects with impaired glucose tolerance (IGT) during a 2-year exercise and dietary intervention. METHODS: Muscle biopsies (musculus vastus lateralis) were taken from 22 subjects belonging to the intervention group of the Finnish Diabetes Prevention Study [1]. According to their myosin heavy chain (MHC) profile at the baseline, the subjects were divided into two groups: IGT(slow) (n=10) with a high proportion of MHC I isoforms and IGT(fast) (n=12) with a high proportion of MHC II isoforms in the vastus lateralis muscle. The intervention consisted of dietary counselling, strength and power training and/or aerobic exercise. The amount of exercise was the same in both groups; the exercise frequency was 5.1+/-2.7 h/week in the IGT(slow) and 5.1+/-2.8 h/week in the IGT(fast) group. RESULTS: Fasting glucose (p<0.05), 2-h glucose (p<0.05), fasting insulin (p<0.05), haemoglobin A1c (HbA(1c)) (p<0.01) and insulin resistance (p<0.05) [homeostasis model assessment for insulin resistance (HOMA-IR)] decreased in the IGT(fast) group, whereas only the 2-h glucose and HbA(1c) concentrations decreased in the IGT(slow) group. The amount of the glycogen synthase kinase-3-alphabeta (GSK-3-alphabeta) decreased in the IGT(fast) group (p<0.05). Exercise training increased the lactate dehydrogenase (LDH) (p<0.01), LDH-1 (p<0.05) and citrate synthase (CS) (p<0.05) activities in the vastus lateralis muscle in the IGT(slow) group, but only the CS activity (p<0.05) in the IGT(fast) group. CONCLUSIONS: The glucose metabolism improved both in the IGT(slow) and IGT(fast) group during the 2-year exercise and dietary intervention. The change was more prominent in the IGT(fast) group than in the IGT(slow) group, associated with the decrease of the GSK-alphabeta protein expression in skeletal muscle. The exercise training improved both glycolytic and oxidative capacity in the vastus lateralis muscle. The glycolytic capacity improved in the IGT(slow) group and the oxidative capacity in both groups.
M Venojärvi; R Puhke; H Hämäläinen; J Marniemi; M Rastas; H Rusko; P Nuutila; O Hänninen; S Aunola
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Publication Detail:
Type:  Journal Article; Multicenter Study; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetes, obesity & metabolism     Volume:  7     ISSN:  1462-8902     ISO Abbreviation:  Diabetes Obes Metab     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-10-12     Completed Date:  2006-04-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100883645     Medline TA:  Diabetes Obes Metab     Country:  England    
Other Details:
Languages:  eng     Pagination:  745-54     Citation Subset:  IM    
Department of Physiology, University of Kuopio, Kuopio, Finland.
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MeSH Terms
Blood Glucose / metabolism
Combined Modality Therapy
Diet, Reducing
Follow-Up Studies
Glucose Intolerance*
Glycogen Synthase Kinase 3 / metabolism
Middle Aged
Muscle Fibers, Skeletal / physiology
Muscle, Skeletal / enzymology,  metabolism,  physiopathology*
Myosin Heavy Chains / metabolism
Obesity / blood*,  physiopathology,  therapy*
Vascular Endothelial Growth Factor A / metabolism
Weight Loss
Reg. No./Substance:
0/Blood Glucose; 0/Myosin Heavy Chains; 0/Vascular Endothelial Growth Factor A; EC Synthase Kinase 3

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