| Role of sialidase in glycoprotein utilisation by Tannerella forsythia. | |
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MedLine Citation:
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PMID: 21885482 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The major bacterial pathogens associated with periodontitis include Tannerella forsythia. We previously discovered that sialic acid stimulates biofilm growth of T. forsythia and that sialidase activity is key to utilisation of sialoconjugate sugars and involved in host-pathogen interactions in vitro. The aim of this work was to assess the influence of the NanH sialidase on initial biofilm adhesion and growth in experiments where the only source of sialic acid was sialo-glycoproteins or human oral secretions. After showing that T. forsythia can utilise sialo-glycoproteins for biofilm growth we showed that growth and initial adhesion with sialylated mucin and fetuin was inhibited 2-3 fold by the sialidase inhibitor oseltamivir. A similar reduction (3-4 fold) was observed with a nanH mutant compared to wild-type. Importantly these data were replicated using clinically relevant serum and saliva samples as substrates. In addition the ability of the nanH mutant to form biofilms on glycoprotein coated surfaces could be restored by the addition of purified NanH, which we show is able to cleave sialic acid from the model glycoprotein fetuin and, much less efficiently, 9-O-acetylated bovine sub-maxillary mucin. These data show for the first time that glycoprotein associated sialic acid is likely to be a key in vivo nutrient source for T. forsythia when growing in a biofilm and suggest that sialidase inhibitors might be useful adjuncts in periodontal therapy. |
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Authors:
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Sumita Roy; Kiyonobu Honma; Ian Douglas; Ashu Sharma; Graham Stafford |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-1 |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: - ISSN: 1465-2080 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of Sheffield; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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