Document Detail

Role of retinol in protecting epithelial cell damage induced by Clostridium difficile toxin A.
MedLine Citation:
PMID:  17825865     Owner:  NLM     Status:  MEDLINE    
Vitamin A (retinol), a fat-soluble vitamin, is an essential nutrient for the normal functioning of the visual system, epithelial cell integrity and growth, immunity, and reproduction. Our group has investigated the effect of high doses of oral vitamin A on early childhood diarrhea in our prospective community-based studies from Northeast Brazil and found a beneficial role in reducing the mean duration but not incidence of diarrheal episodes. In this study, we explored the role of retinol supplementation in intestinal cell lines following Clostridium difficile toxin A (TxA) challenge. C. difficile is the most common anaerobic pathogen borne with antibiotic-borne diarrhea and pseudomembranous colitis. Since retinol is critical for the integrity of tight junctions and to modulate the cell cycle, we have focused on changes in transepithelial electrical resistance (TEER) in Caco-2, a more differentiated intestinal cell line, and on models of cell proliferation, migration and viability in IEC-6 cells, an undifferentiated crypt cell line, following TxA injury. In this model, retinol therapy reduced apoptosis, improved cell migration and proliferation, and prevented the reduction in TEER, following C. difficile TxA challenge in a glutamine-free medium. These results suggest the role of retinol in protecting intestinal epithelial barrier function from C. difficile TxA enterotoxic damage.
Andressa A F L Maciel; Reinaldo B Oriá; Manuel B Braga-Neto; Andréa B Braga; Eunice B Carvalho; Herene B M Lucena; Gerly A C Brito; Richard L Guerrant; Aldo A M Lima
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-07-31
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  50     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-20     Completed Date:  2008-03-25     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  1027-40     Citation Subset:  IM    
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MeSH Terms
Bacterial Toxins / toxicity*
Caco-2 Cells
Cell Movement / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Electric Impedance
Enterotoxins / toxicity*
Epithelial Cells / drug effects
Flow Cytometry
Vitamin A / pharmacology*
Grant Support
D43 TW006578/TW/FIC NIH HHS; D43 TW006578-04S1/TW/FIC NIH HHS; D43 TW006578-04S1/TW/FIC NIH HHS; HD053131-01/HD/NICHD NIH HHS; U01 AI026512-15S2/AI/NIAID NIH HHS
Reg. No./Substance:
0/Bacterial Toxins; 0/Enterotoxins; 0/tcdA protein, Clostridium difficile; 11103-57-4/Vitamin A

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