Document Detail

Role of renal aquaporins in escape from vasopressin-induced antidiuresis in rat.
MedLine Citation:
PMID:  9109429     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to investigate whether escape from vasopressin-induced antidiuresis is associated with altered regulation of any of the known aquaporin water channels. After 4-d pretreatment with 1-deamino-[8-D-arginine]-vasopressin (dDAVP) by osmotic mini-pump, rats were divided into two groups: control (continued dDAVP) and water-loaded (continued dDAVP plus a daily oral water load). A significant increase in urine volume in the water-loaded rats was observed by the second day of water loading, indicating onset of vasopressin escape. The onset of escape coincided temporally with a marked decrease in renal aquaporin-2 protein (measured by semiquantitative immunoblotting), which began at day 2 and fell to 17% of control levels by day 3. In contrast, there was no decrease in the renal expression of aquaporins 1, 3, or 4. The marked suppression of whole kidney aquaporin-2 protein was accompanied by a concomitant suppression of whole kidney aquaporin-2 mRNA levels. Immunocytochemical localization and differential centrifugation studies demonstrated that trafficking of aquaporin-2 to the plasma membrane remained intact during vasopressin escape. The results suggest that escape from vasopressin-induced antidiuresis is attributable, at least in part, to a vasopressin-independent decrease in aquaporin-2 water channel expression in the renal collecting duct.
C A Ecelbarger; S Nielsen; B R Olson; T Murase; E A Baker; M A Knepper; J G Verbalis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  99     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-09     Completed Date:  1997-05-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1852-63     Citation Subset:  AIM; IM    
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
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MeSH Terms
Aquaporin 2
Aquaporin 3
Aquaporin 6
Base Sequence
Cell Membrane Permeability / drug effects,  physiology
DNA Primers / genetics
Deamino Arginine Vasopressin / pharmacology*
Diuresis / drug effects*,  physiology*
Ion Channels / genetics,  physiology*
Kidney / drug effects*,  physiology*
Kidney Tubules, Collecting / drug effects,  physiology
RNA, Messenger / genetics,  metabolism
Rats, Sprague-Dawley
Renal Agents / pharmacology
Water / metabolism
Grant Support
Reg. No./Substance:
0/Aqp2 protein, rat; 0/Aqp3 protein, rat; 0/Aquaporin 2; 0/Aquaporin 6; 0/Aquaporins; 0/DNA Primers; 0/Ion Channels; 0/RNA, Messenger; 0/Renal Agents; 158801-98-0/Aquaporin 3; 16679-58-6/Deamino Arginine Vasopressin; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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