Document Detail


Role of Unc104/KIF1-related motor proteins in mitochondrial transport in Neurospora crassa.
MedLine Citation:
PMID:  15483054     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eukaryotic cells use diverse cytoskeleton-dependent machineries to control inheritance and intracellular positioning of mitochondria. In particular, microtubules play a major role in mitochondrial motility in the filamentous fungus Neurospora crassa and in mammalian cells. We examined the role of two novel Unc104/KIF1-related members of the kinesin family, Nkin2 and Nkin3, in mitochondrial motility in Neurospora. The Nkin2 protein is required for mitochondrial interactions with microtubules in vitro. Mutant hyphae lacking Nkin2 show mitochondrial motility defects in vivo early after germination of conidiospores. Nkin3, a member of a unique fungal-specific subgroup of small Unc104/KIF1-related proteins, is not associated with mitochondria in wild-type cells. However, it is highly expressed and recruited to mitochondria in Deltankin-2 mutants. Mitochondria lacking Nkin2 require Nkin3 for binding to microtubules in vitro, and mitochondrial motility defects in Deltankin-2 mutants disappear with up-regulation of Nkin3 in vivo. We propose that mitochondrial transport is mediated by Nkin2 in Neurospora, and organelle motility defects in Deltankin-2 mutants are rescued by Nkin3. Apparently, a highly versatile complement of organelle motors allows the cell to efficiently respond to exogenous challenges, a process that might also account for the great variety of different mitochondrial transport systems that have evolved in eukaryotic cells.
Authors:
Florian Fuchs; Benedikt Westermann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-10-13
Journal Detail:
Title:  Molecular biology of the cell     Volume:  16     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-22     Completed Date:  2005-07-06     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-61     Citation Subset:  IM    
Affiliation:
Institut für Physiologische Chemie, Universität München, 81377 München, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Caenorhabditis elegans Proteins / metabolism,  physiology*
Cell Movement
Cell Proliferation
Microscopy
Microtubules / metabolism,  ultrastructure
Mitochondria / metabolism*
Mutation
Nerve Tissue Proteins / metabolism,  physiology*
Neurospora crassa / metabolism*
Phylogeny
Plasmids / metabolism
Protein Binding
Subcellular Fractions
Time Factors
Up-Regulation
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Nerve Tissue Proteins; 0/UNC-104 protein, C elegans
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