Document Detail

Role of reactive oxygen species and phosphatidylinositol 3-kinase in cardiomyocyte differentiation of embryonic stem cells.
MedLine Citation:
PMID:  10913617     Owner:  NLM     Status:  MEDLINE    
Cardiotypic development in embryonic stem cell-derived embryoid bodies may be regulated by reactive oxygen species (ROS). ROS were generated by a NADPH oxidase-like enzyme which was transiently expressed during the time course of embryoid body development. Incubation with either H(2)O(2) or menadione enhanced cardiomyogenesis, whereas the radical scavengers trolox, pyrrolidinedithiocarbamate and N-acetylcysteine exerted inhibitory effects. The phosphatidylinositol 3-kinase (PI-3-kinase) inhibitors LY294002 and wortmannin abolished cardiac commitment and downregulated ROS in embryoid bodies. Coadministration of LY294002 with prooxidants resumed cardiomyocyte differentiation, indicating a role for PI-3-kinase in the regulation of the intracellular redox state.
H Sauer; G Rahimi; J Hescheler; M Wartenberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  476     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-08-16     Completed Date:  2000-08-16     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  218-23     Citation Subset:  IM    
Department of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, D-50931, Cologne, Germany.
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MeSH Terms
1-Phosphatidylinositol 3-Kinase / antagonists & inhibitors,  metabolism*
Androstadienes / pharmacology
Cell Differentiation / drug effects
Cell Line
Chromones / pharmacology
Enzyme Inhibitors / pharmacology
Fetal Heart / cytology,  drug effects,  metabolism
Hydrogen Peroxide / pharmacology
Morpholines / pharmacology
Myocardium / cytology*,  metabolism*
NADPH Oxidase / metabolism
Oxidants / pharmacology
Reactive Oxygen Species / metabolism*
Signal Transduction
Stem Cells / cytology,  drug effects,  metabolism
Vitamin K / pharmacology
Reg. No./Substance:
0/Androstadienes; 0/Chromones; 0/Enzyme Inhibitors; 0/Morpholines; 0/Oxidants; 0/Reactive Oxygen Species; 12001-79-5/Vitamin K; 154447-36-6/2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; 19545-26-7/wortmannin; 7722-84-1/Hydrogen Peroxide; EC Oxidase; EC 3-Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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