Document Detail

Role of reactive oxygen intermediates in lipopolysaccharide-mediated hepatic injury in the rat.
MedLine Citation:
PMID:  8592424     Owner:  NLM     Status:  MEDLINE    
Because reactive oxygen intermediates derived from xanthine oxidase may have an important role in the pathophysiology of lipopolysaccharide-mediated tissue injury, we studied hydrogen peroxide generation using 3-amino-1,2,4-triazole inactivation of hepatic catalase and the ratio of xanthine oxidase to xanthine dehydrogenase activity in rat livers after in vivo lipopolysaccharide administration. We also studied the effect of tungsten, a potent inhibitor of xanthine oxidase, on the toxicity of lipopolysaccharide. There was increased hydrogen peroxide production and enhanced proteolytic conversion from xanthine dehydrogenase to xanthine oxidase in rat livers after lipopolysaccharide administration. Feeding rats a tungsten-rich diet for 4 weeks greatly diminished hepatic xanthine oxidase activity and lessened the rise in intracellular hydrogen peroxide production after lipopolysaccharide treatment. Liver damage, as assessed by the serum transaminase levels and mortality, was also ameliorated by the tungsten-rich diet. These findings suggest that hydrogen peroxide derived from xanthine oxidase contributes to the development of systemic toxicity and liver damage after lipopolysaccharide administration.
N Takeyama; Y Shoji; K Ohashi; T Tanaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  60     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-04-03     Completed Date:  1996-04-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  258-62     Citation Subset:  IM    
Department of Emergency and Critical Care Medicine, Kansai Medical University, Osaka, Japan.
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MeSH Terms
Adenosine / metabolism
Drug-Induced Liver Injury
Enzymes / blood
Hydrogen Peroxide / metabolism
Lipopolysaccharides / pharmacology*
Liver Diseases / enzymology,  metabolism*
Rats, Wistar
Reactive Oxygen Species / metabolism*
Tungsten / administration & dosage
Xanthine Dehydrogenase / metabolism
Xanthine Oxidase / metabolism
Reg. No./Substance:
0/Enzymes; 0/Lipopolysaccharides; 0/Reactive Oxygen Species; 58-61-7/Adenosine; 7440-33-7/Tungsten; 7722-84-1/Hydrogen Peroxide; EC Dehydrogenase; EC Oxidase

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