Document Detail


Role of protein kinase Cbeta in rhythmic contractions of human pregnant myometrium.
MedLine Citation:
PMID:  17504923     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess the role of protein kinase Cbeta (PKCbeta) in human myometrial contractions during pregnancy, we evaluated the effect of a PKCbeta inhibitor (LY333531) on the pregnant and nonpregnant myometrial contractions and compared the level of PKCbeta in the pregnant myometrium with that in the nonpregnant myometrium. The effects of LY333531 on the myometrial contractions were examined by measuring contractile activity (frequency and amplitude). PKCbeta in human myometrium was assessed at mRNA level using real-time PCR method. The characteristics of contractile activity were different between the pregnant and the nonpregnant myometrium. The amplitude of rhythmic contractions in the preterm and term myometrium was increased 2- to 2.5-fold when compared with that in the nonpregnant myometrium, but the frequency of rhythmic contractions was decreased by about half. LY333531 (10(-6) M) reduced the increased amplitude in the preterm and term myometrium by about 50%, and the inhibitory effects of LY333531 in the pregnant myometrium were significantly greater than that in the nonpregnant myometrium (about 50 vs 25%). However, the frequency in the pregnant and nonpregnant myometrium was not influenced by LY333531. Real-time PCR revealed a significant, five- to sevenfold increase in the expression of PKCbeta mRNA in the preterm and term myometrium when compared with the nonpregnant myometrium. These findings suggest that the increased amplitude of human myometrial contractions during pregnancy is related to the increased level of PKCbeta. A PKCbeta inhibitor may reduce preterm uterine contractions and prevent preterm delivery.
Authors:
Katsuhiko Yasuda; Tsuyoshi Nakamoto; Masahiro Yasuhara; Hidetaka Okada; Tatsuya Nakajima; Hideharu Kanzaki; Masatoshi Hori; Hiroshi Ozaki
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  133     ISSN:  1470-1626     ISO Abbreviation:  Reproduction     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-05-16     Completed Date:  2007-09-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  797-806     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8507, Japan. yasuda@takii.kmu.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adult
Carbazoles / pharmacology
Case-Control Studies
Enzyme Inhibitors / pharmacology
Female
Humans
Indoles / pharmacology
Labor, Obstetric / physiology*
Maleimides / pharmacology
Myometrium / enzymology,  physiology*
Obstetric Labor, Premature / enzymology
Oxytocics / pharmacology
Oxytocin / pharmacology
Pregnancy
Pregnancy Trimester, Third
Protein Kinase C / antagonists & inhibitors,  physiology*
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction / methods
Stimulation, Chemical
Uterine Contraction / drug effects,  physiology*
Chemical
Reg. No./Substance:
0/Carbazoles; 0/Enzyme Inhibitors; 0/Indoles; 0/Maleimides; 0/Oxytocics; 0/RNA, Messenger; 125314-64-9/Ro 31-8220; 136194-77-9/Go 6976; 169939-94-0/ruboxistaurin; 50-56-6/Oxytocin; EC 2.7.1.-/protein kinase C beta; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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