Document Detail

Role of platelet activating factor in acute pancreatitis induced by lipopolysaccharides in rabbits.
MedLine Citation:
PMID:  7813547     Owner:  NLM     Status:  MEDLINE    
In the present study we demonstrated that a single injection of endotoxin (lipopolysaccharides, E. Coli 0111-B4) into the superior pancreaticoduodenal artery of rabbits induced a dose-dependent acute necrotizing pancreatitis. The lesions observed by light microscopy were significant for 10 micrograms lipopolysaccharides and were maximal for 20 micrograms. After 24 h the main findings were edema, acinar cell vacuolisation, polymorphonuclear neutrophil infiltration and tissue necrosis. The pancreatic lesions developed strictly in the area supplied by the artery injected with lipopolysaccharides, without significant intestinal involvement. Since platelet-activating factor (1-O-hexadecyl-2-acetyl-sn-glycero-3- phosphocholine, PAF; 50-500 ng), a phospholipid mediator of endotoxin-induced inflammation and shock, was previously shown to cause an acute necrotizing pancreatitis in rabbits, the role of PAF in the development of acute pancreatitis induced by lipopolysaccharides was studied by evaluating: (1) the synergism between doses of lipopolysaccharides (5-10 micrograms), which produced a mild tissue injury, and doses of PAF (10 ng) not producing, per se, any significant injury, and (2) the effect of three structurally unrelated PAF receptor antagonists. The results obtained demonstrated that 10 ng of PAF significantly potentiated pancreatic tissue damage induced by 10 micrograms of lipopolysaccharides.(ABSTRACT TRUNCATED AT 250 WORDS)
G Emanuelli; G Montrucchio; L Dughera; E Gaia; E Lupia; E Battaglia; A De Martino; P De Giuli; L Gubetta; G Camussi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmacology     Volume:  261     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1995-02-09     Completed Date:  1995-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  265-72     Citation Subset:  IM    
Dipartimento di Fisiopatologia Clinica, Università di Torino, Italy.
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MeSH Terms
Acute Disease
Escherichia coli*
Pancreatitis / chemically induced,  pathology*
Platelet Activating Factor / antagonists & inhibitors,  physiology*
Platelet Membrane Glycoproteins / antagonists & inhibitors
Receptors, Cell Surface*
Receptors, G-Protein-Coupled*
Reg. No./Substance:
0/Lipopolysaccharides; 0/Platelet Activating Factor; 0/Platelet Membrane Glycoproteins; 0/Receptors, Cell Surface; 0/Receptors, G-Protein-Coupled; 0/platelet activating factor receptor

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