Document Detail


Role of pituitary tumour transforming gene 1 in medullary thyroid carcinoma.
MedLine Citation:
PMID:  20978326     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: pituitary tumour transforming gene 1 (PTTG1) is over-expressed in a variety of endocrine-related tumours. We aimed at evaluating PTTG1 expression and function in human neoplastic parafollicular C-cells, represented by medullary thyroid carcinoma (MTC) and C-cell hyperplasia (CCH) samples and by the TT cell line.
METHODS: TT cells and tissues derived from human CCH (8 samples) and MTC (12 samples) were analyzed by northern blot, furthermore TT cells were subjected to PTTG gene silencing and cells were analyzed for DNA synthesis.
RESULTS: PTTG1 expression was significantly higher (p<0.01) in CCH (3-fold), in papillary thyroid cancer and in MTC (5-fold) than in normal thyroid, and in MTC lymph-node metastases as compared to primary lesions (approximately 2-fold; p<0.05). PTTG1 mRNA and protein correlated with tumour diameter and TNM status (p<0.05). In TT cells, PTTG1 silencing did not completely block DNA synthesis, but significantly reduced [3H]Thymidine incorporation (~50%; p<0.01) for up to 3 days.
CONCLUSIONS: PTTG1 levels correlate with tumour aggressiveness. PTTG1 silencing causes reduced MTC cell proliferation, supporting the hypothesis that PTTG1 might have an important role in C-cell neoplastic proliferation.
Authors:
Maria Chiara Zatelli; Federico Tagliati; Vincenzo Amodio; Mattia Buratto; Mariarosa Pelizzo; Giancarlo Pansini; Marta Bondanelli; Maria Rosaria Ambrosio; Ettore C Degli Uberti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical cellular pathology (Amsterdam)     Volume:  33     ISSN:  2210-7185     ISO Abbreviation:  Anal Cell Pathol (Amst)     Publication Date:  2010  
Date Detail:
Created Date:  2011-01-20     Completed Date:  2011-04-27     Revised Date:  2011-06-02    
Medline Journal Info:
Nlm Unique ID:  101541993     Medline TA:  Anal Cell Pathol (Amst)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  207-16     Citation Subset:  IM    
Affiliation:
Section of Endocrinology, Department of Biomedical Sciences and Advanced Therapies, University of Ferrara, Ferrara, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Blotting, Northern
Blotting, Western
Cell Proliferation
Child
Female
Gene Silencing
Humans
Hyperplasia
Lymphatic Metastasis
Male
Microscopy, Fluorescence
Middle Aged
Neoplasm Proteins / biosynthesis*,  genetics
Neoplasm Staging
Reverse Transcriptase Polymerase Chain Reaction
Thyroid Neoplasms / genetics,  metabolism,  pathology
Tumor Markers, Biological / analysis*,  genetics,  metabolism
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/Tumor Markers, Biological; 0/pituitary tumor-transforming proteins

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