Document Detail


Role of pilus proteins in adherence and invasion of Streptococcus agalactiae to the lung and cervical epithelial cells.
MedLine Citation:
PMID:  23209289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Streptococcus agalactiae, or group B Streptococcus (GBS), is an important opportunistic pathogen that causes pneumonia, sepsis, and meningitis in neonates and severe diseases in immunocompromised adults. We have performed comparative genomics of prevalent GBS serotypes of Indian origin (i.e. Ia, III, V, and VII). Pilus-proteins were commonly found up-regulated, and their expression was studied by using antiserum for GBS80 (backbone protein of pilus island-I), GBS67 (ancillary protein of PI-2a), and SAN1518 (backbone protein of PI-2b) by whole cell and Western blot analysis. To check the role of pilus proteins in adherence and invasion, an inhibition assay was performed. Comparative immunoblotting experiments revealed that expression of pili proteins does not differ in geographically different selected serotypes, Ia and V, of India and the United States. In the case of A549 cells, we found that GBS VII invasion and adherence was inhibited by pilus protein-specific antiserum SAN1518 significantly (p < 0.001) by 88.5 and 91%, respectively. We found that mutant strains, deficient in the pilus proteins (Δgbs80 and Δsan1518) exhibit a significant decrease in adherence in the case of type Ia, III, and VII. In the case of type VII, we have found a 95% reduction in invasion when Δsan1518 was used with A549 cells. Because the pilus proteins were identified previously as vaccine candidates against GBS serotypes of developed countries, we also found their role in the attachment and invasion of GBS of Indian origin. Thus, the present work supports the idea of making a more effective pilus protein-based vaccine that can be used universally.
Authors:
Puja Sharma; Hem Lata; Deepak Kumar Arya; Arun Kumar Kashyap; Hemant Kumar; Meenakshi Dua; Arif Ali; Atul Kumar Johri
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-03
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  288     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-11     Completed Date:  2013-04-19     Revised Date:  2014-02-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4023-34     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Bacterial Adhesion*
Bacterial Proteins / genetics,  metabolism*
Cell Line
Cervix Uteri / metabolism*,  microbiology,  pathology
Epithelial Cells / metabolism*,  microbiology,  pathology
Female
Fimbriae, Bacterial / genetics,  metabolism*
Humans
India
Lung / metabolism*,  microbiology,  pathology
Male
Streptococcal Infections / genetics,  metabolism*,  pathology,  prevention & control
Streptococcal Vaccines / genetics,  therapeutic use
Streptococcus agalactiae / genetics,  metabolism*,  pathogenicity
United States
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Streptococcal Vaccines
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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