| Role of pili proteins in adherence and invasion of Streptococcus agalactiae to the lung and cervical epithelial cells. | |
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MedLine Citation:
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PMID: 23209289 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Streptococcus agalactiae or GBS is an important opportunistic pathogen which causes pneumonia, sepsis and meningitis in neonates and severe diseases in immunocompromised adults. We have performed comparative genomics of prevalent GBS serotypes of Indian origin i.e. Ia III V and VII. Pili proteins were commonly found upregulated and their expression was studied by using antiserum for GBS80 (backbone protein of pilus islandI (PI1), GBS67 (ancillary protein of PI2a) and SAN1518 (backbone protein of PI2b) by whole cell and Western blot analysis. To check the role of pili proteins in adherence and invasion, inhibition assay was performed. Comparative immunoblotting experiments revealed that pili proteins expression does not differ in geographically different selected serotypes, Ia and V of India and USA. In case of A549 we found that GBS VII invasion and adherence was inhibited by pili protein specific antiserum SAN1518 significantly (P=<0.001) by 88.5 and 91 % respectively. We found that mutant strains, deficient in the pilus proteins (Δgbs80, and Δsan1518 exhibit a significant decrease in adherence in case of type Ia, III, VII. We have found 95 % reduction in invasion when SAN1518 was used in case of A549 cells. As the pili proteins were identified previously as vaccine candidates against GBS serotypes of developed countries and we also found their role in the attachment and invasion of GBS of Indian origin thus in turn present work supports the idea of making a more effective pili protein based vaccine which can be used universally. |
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Authors:
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Puja Sharma; Hem Lata; Deepak Kumar Araya; Arun Kumar Kashyap; Hemant Kumar Pathak; Meenakshi Dua; Arif Ali; Atul Kumar Johri |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-4 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: - ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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School of Life Sciences, Jawaharlal Nehru University, India; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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