Document Detail


Role for phosphatidylinositol in nuclear envelope formation.
MedLine Citation:
PMID:  11368777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PtdIns is a minor membrane phospholipid that is important in signal transduction. Recently, derivatives of PtdIns phosphorylated at the 3-position of the inositol ring have been implicated in the regulation of constitutive membrane traffic and in membrane fusion events. Assembly of the nuclear envelope (NE), a crucial step in the progress of mitosis, is also likely to involve membrane fusion reactions. We therefore investigated the role of PtdIns and phosphoinositide 3-kinase (PI-3K) activity in NE formation in vitro. GTP-induced NE formation was blocked by wortmannin and LY294002, two specific inhibitors of PI-3K, suggesting a role for PtdIns phosphorylated at the 3-position. PtdIns-specific phospholipase C mimicked GTP hydrolysis as an inducer of NE formation. This induction was dependent on a membrane vesicle subfraction (MV1) that was highly enriched in PtdIns, as determined by heteronuclear two-dimensional NMR spectroscopy. On the basis of these results, we suggest that the MV1 population serves as a source of membranes rich in PtdIns that might facilitate fusion, possibly through the production of the membrane-destabilizing lipid diacylglycerol.
Authors:
B Larijani; T M Barona; D L Poccia
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  356     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-22     Completed Date:  2001-07-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  495-501     Citation Subset:  IM    
Affiliation:
Department of Biology, Amherst College, Amherst, MA 01002, USA. b.larijani@icrf.icnet.uk
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MeSH Terms
Descriptor/Qualifier:
1-Phosphatidylinositol 3-Kinase / antagonists & inhibitors,  metabolism
Androstadienes / pharmacology
Animals
Chromones / pharmacology
Female
Guanosine Triphosphate / pharmacology
Male
Membrane Fusion / drug effects
Morpholines / pharmacology
Nuclear Envelope / drug effects,  metabolism*
Phosphatidylinositol Diacylglycerol-Lyase
Phosphatidylinositols / metabolism*
Phospholipids / metabolism
Sea Urchins
Type C Phospholipases / pharmacology
Grant Support
ID/Acronym/Agency:
GM-55970/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Androstadienes; 0/Chromones; 0/Morpholines; 0/Phosphatidylinositols; 0/Phospholipids; 154447-36-6/2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; 19545-26-7/wortmannin; 86-01-1/Guanosine Triphosphate; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 3.1.4.-/Type C Phospholipases; EC 4.6.1.13/Phosphatidylinositol Diacylglycerol-Lyase
Comments/Corrections

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