| Role for phosphatidylinositol in nuclear envelope formation. | |
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MedLine Citation:
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PMID: 11368777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PtdIns is a minor membrane phospholipid that is important in signal transduction. Recently, derivatives of PtdIns phosphorylated at the 3-position of the inositol ring have been implicated in the regulation of constitutive membrane traffic and in membrane fusion events. Assembly of the nuclear envelope (NE), a crucial step in the progress of mitosis, is also likely to involve membrane fusion reactions. We therefore investigated the role of PtdIns and phosphoinositide 3-kinase (PI-3K) activity in NE formation in vitro. GTP-induced NE formation was blocked by wortmannin and LY294002, two specific inhibitors of PI-3K, suggesting a role for PtdIns phosphorylated at the 3-position. PtdIns-specific phospholipase C mimicked GTP hydrolysis as an inducer of NE formation. This induction was dependent on a membrane vesicle subfraction (MV1) that was highly enriched in PtdIns, as determined by heteronuclear two-dimensional NMR spectroscopy. On the basis of these results, we suggest that the MV1 population serves as a source of membranes rich in PtdIns that might facilitate fusion, possibly through the production of the membrane-destabilizing lipid diacylglycerol. |
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Authors:
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B Larijani; T M Barona; D L Poccia |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Biochemical journal Volume: 356 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-05-22 Completed Date: 2001-07-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: England |
Other Details:
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Languages: eng Pagination: 495-501 Citation Subset: IM |
Affiliation:
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Department of Biology, Amherst College, Amherst, MA 01002, USA. b.larijani@icrf.icnet.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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antagonists & inhibitors,
metabolism Androstadienes / pharmacology Animals Chromones / pharmacology Female Guanosine Triphosphate / pharmacology Male Membrane Fusion / drug effects Morpholines / pharmacology Nuclear Envelope / drug effects, metabolism* Phosphatidylinositol Diacylglycerol-Lyase Phosphatidylinositols / metabolism* Phospholipids / metabolism Sea Urchins Type C Phospholipases / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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GM-55970/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Androstadienes; 0/Chromones; 0/Morpholines; 0/Phosphatidylinositols; 0/Phospholipids; 154447-36-6/2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; 19545-26-7/wortmannin; 86-01-1/Guanosine Triphosphate; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 3.1.4.-/Type C Phospholipases; EC 4.6.1.13/Phosphatidylinositol Diacylglycerol-Lyase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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