| Role of the pfcrt codon 76 mutation as a molecular marker for population-based surveillance of chloroquine (CQ)-resistant Plasmodium falciparum malaria in Ugandan sentinel sites with high CQ resistance. | |
| | |
MedLine Citation:
|
PMID: 12474488 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The mutant genotype at codon 76 of the pfcrt gene (T76) has been proposed as a molecular marker for surveillance of chloroquine (CQ)-resistant Plasmodium falciparum malaria but this proposal has not been validated by population-based surveys. In 1998-99, in 6 Ugandan sentinel sites, the prevalence of P. falciparum infections with the T76 genotype and the level of CQ use were measured by community surveys, and CQ resistance was determined by in-vivo tests on 6-59-month-old children with clinical malaria. The prevalence of T76 was not related to the overall clinical (early and late treatment failure: ETF + LTF; r = 0.14, P = 0.78) or parasitological (RI + RII + RIII; r = 0.17, P = 0.73) CQ resistance. However, the percentage of individuals carrying only infections with the T76 genotype (T76 alone) increased with increasing ETF (r = 0.76, P = 0.07) and type RIII parasitological failure (r = 0.69, P = 0.12). Similarly, the ratio between T76 and K76 (the wild type) prevalences (T76/K76) was strongly and positively correlated with ETF (r = 0.85, P = 0.03) and RIII (r = 0.82, P = 0.04). Moreover, T76 alone (r = 0.90, P = 0.01) as well as T76/K76 (r = 0.90, P = 0.01) significantly increased with increasing community CQ use. T76 alone and T76/K76 can be useful markers to estimate the ETF and RIII prevalence as well as the amount of CQ use in the community. |
| | |
Authors:
|
Ambrose O Talisuna; Jackie Kyosiimire-Lugemwa; Peter Langi; Theonest K Mutabingwa; William Watkins; Eric Van Marck; Thomas Egwang; Umberto D'Alessandro |
Related Documents
:
|
7844398 - Malaria in east african refugees resettling to the united states: development of strate... 1510648 - Crewmember communication in space: a survey of astronauts and cosmonauts. 9653728 - "reasons for contentment": malaria in india, 1900-1920. 17849898 - Effects of proportion and configuration of bacillus thuringiensis cotton on pest abunda... 2682248 - Problems in application of the terms 'blastic' and 'thallic' to modes of conidiogenesis... 7087418 - Evaluation of high-fidelity hearing aids. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Transactions of the Royal Society of Tropical Medicine and Hygiene Volume: 96 ISSN: 0035-9203 ISO Abbreviation: Trans. R. Soc. Trop. Med. Hyg. Publication Date: 2002 Sep-Oct |
Date Detail:
|
Created Date: 2002-12-11 Completed Date: 2003-01-03 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7506129 Medline TA: Trans R Soc Trop Med Hyg Country: England |
Other Details:
|
Languages: eng Pagination: 551-6 Citation Subset: IM |
Affiliation:
|
Ministry of Health, P.O. Box 7272, Kampala, Uganda. atalisuna@kla1.afsat.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Child, Preschool Chloroquine / therapeutic use* Drug Resistance / genetics Genetic Markers Genotype Humans Infant Malaria, Falciparum / drug therapy, epidemiology* Membrane Proteins / genetics* Membrane Transport Proteins Mutation / genetics* Plasmodium falciparum / drug effects, genetics* Prevalence Protozoan Proteins Uganda / epidemiology |
| Chemical | |
Reg. No./Substance:
|
0/Genetic Markers; 0/Membrane Proteins; 0/Membrane Transport Proteins; 0/PfCRT protein, Plasmodium falciparum; 0/Protozoan Proteins; 54-05-7/Chloroquine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: High rate of Bartonella henselae infection in HIV-positive outpatients in Johannesburg, South Africa...
Next Document: Current absence of pyrimethamine resistance of Plasmodium falciparum in Madagascar.