Document Detail


Role of the ompT mutation in stimulated decrease in colony-forming ability due to intracellular protein aggregate formation in Escherichia coli strain BL21.
MedLine Citation:
PMID:  17284844     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently we found that the cells of Escherichia coli strain BL21 producing a fusion protein, GST-Sup35NM, show a much more rapid decrease in colony-forming ability in the stationary phase than control cells. In this study, it was found that an extract of the cells producing GST-Sup35NM forms fibrous protein polymers containing GST-Sup35NM. In the course of the study, we realized that strain BL21 carried the ompT mutation. We suspected that the deficiency in OmpT protease was responsible for the observed phenotype. To test this, we introduced the wild-type ompT gene into strain BL21, and found that the transformed cells recovered the wild-type phenotype. We concluded that OmpT protease, though known to localize on the cell surface, is involved in protein quality control within the cell.
Authors:
Bun-ichiro Ono; Hiroko Kimiduka; Masashi Kubota; Kazuaki Okuno; Masayuki Yabuta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  71     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-23     Completed Date:  2007-05-09     Revised Date:  2010-06-17    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  504-12     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga, Japan. ono@se.ritsumei.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Bacterial Outer Membrane Proteins / genetics*
Bacterial Proteins / biosynthesis*,  chemistry,  genetics*
Blotting, Western
Centrifugation, Density Gradient
Colony Count, Microbial
Congo Red
Culture Media
DNA, Bacterial / chemistry,  genetics
Electrophoresis, Polyacrylamide Gel
Escherichia coli / genetics*,  growth & development,  metabolism*
Escherichia coli Proteins / genetics*
Glutathione / metabolism
Glycerol / chemistry
Microscopy, Electron, Scanning
Mutation / genetics
Peptide Hydrolases / genetics*
Plasmids / genetics
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Culture Media; 0/DNA, Bacterial; 0/Escherichia coli Proteins; 0/ompT protein, E coli; 56-81-5/Glycerol; 573-58-0/Congo Red; 70-18-8/Glutathione; EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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