Document Detail

Role of nuclear size in cell growth initiation.
MedLine Citation:
PMID:  451539     Owner:  NLM     Status:  MEDLINE    
Swiss 3T3 cells arrested in B0 (quiescent state) by reducing serum content of the medium all contain the same amount of DNA but vary in nuclear volume over approximately a twofold range. By use of flow microfluorimetry, scatterplots of nuclear volume versus DNA content were obtained in intervals after serum stimulation. The earliest cells to enter DNA synthesis were those with the largest nuclei, whereas cells with the smallest nuclei were among the latest. Regulation of cellular transit from G0 to the S phase was therefore, at least in part, deterministic, since all G0 cells did not have equal probabilities of entry into S at a given moment. All cells having the same nuclear volume did not initiate DNA synthesis at the same moment; therefore, factors other than nuclear volume must also influence this timing. Nuclear volume correlated with the maximum rate at which cells could enter S. The kinetic model of the cell cycle postulating a probabilistic event as solely responsible for entry into S thus appears too simple.
A Yen; A B Pardee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  204     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1979 Jun 
Date Detail:
Created Date:  1979-08-29     Completed Date:  1979-08-29     Revised Date:  2007-03-19    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1315-7     Citation Subset:  IM    
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MeSH Terms
Cell Cycle*
Cell Division
Cell Nucleus / physiology,  ultrastructure*
Cells, Cultured / physiology*,  ultrastructure
Clone Cells / ultrastructure
DNA / biosynthesis
Reg. No./Substance:

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