Document Detail

Role of nonclassical class I genes of the chicken major histocompatibility complex Rfp-Y locus in transplantation immunity.
MedLine Citation:
PMID:  14597999     Owner:  NLM     Status:  MEDLINE    
The chicken major histocompatibility complex ( MHC) genes are organized into two genetically independent clusters which both possess class I and class IIbeta genes: the classical B complex and the Restriction fragment pattern- Y ( Rfp-Y) complex. In this study, we have examined the role of Rfp-Y genes in transplantation immunity. For this we used three sublines, B19H1, B19H2 and B19H3, derived from a line fixed for B19. Southern blots, PCR-SSCP assays using primers specific for Rfp-Y genes, and Rfp-Y class I allele-specific sequencing show that the polymorphisms observed in B19H1, B19H2 and B19H3 are due to the presence of three different Rfp-Y haplotypes. The Rfp-Y class I ( YF) alleles in these three haplotypes are highly polymorphic, and RT-PCR shows that at least two YF loci are expressed in each subline. The three sublines show Rfp-Y-directed alloreactivity in that Rfp-Y-incompatible skin grafts are rejected within 15 days, a rate intermediate between that seen in B-incompatible rejection (7 days) and that observed for grafts within the sublines (20 days). We conclude that Rfp-Y has an intermediate role in allograft rejection, likely to be attributable to polymorphism at the class I loci within this region.
Pierrick Thoraval; Marielle Afanassieff; Danièle Bouret; Gillette Luneau; Evelyne Esnault; Ronald M Goto; Anne-Marie Chaussé; Rima Zoorob; Denis Soubieux; Marcia M Miller; Ginette Dambrine
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-11-04
Journal Detail:
Title:  Immunogenetics     Volume:  55     ISSN:  0093-7711     ISO Abbreviation:  Immunogenetics     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-15     Completed Date:  2004-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0420404     Medline TA:  Immunogenetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  647-51     Citation Subset:  IM    
Unité 086, Bioagresseurs, Santé, Environnement, INRA, Centre de Tours-Nouzilly, 37380, Nouzilly, France.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY257165;  AY257166;  AY257167;  AY257168;  AY257169;  AY257170
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MeSH Terms
Amino Acid Sequence
Blotting, Southern
Chickens / genetics*,  immunology
Crosses, Genetic
Graft Rejection / immunology
Major Histocompatibility Complex / genetics*
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Restriction Mapping
Sequence Homology, Amino Acid
Skin Transplantation / immunology
Transplantation Immunology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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