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Role of the non-canonical Notch ligand DLK1 in hormone-producing cells of the adult male mouse pituitary.
MedLine Citation:
PMID:  21756269     Owner:  NLM     Status:  Publisher    
To better understand the role of the non-canonical Notch ligand delta-like protein 1 (DLK1), in hormone-producing cells, we studied the cell distribution and subcellular localisation of DLK1 in the pituitary of male adult 129/SvJ mice, and analysed the variations in the hormone-producing cells associated with the lack of this gene in Dlk1 knockout mice. Our results showed the presence of DLK1-immunoreactive cells (DLK1-ir) in all hormone-producing cells of the anterior pituitary. Immunoelectron microscopy showed DLK1-ir in the rough endoplasmic reticulum and inside secretory vesicles, suggesting that DLK1 is released together with pituitary hormones. Moreover, we found that PRL-DLK1-ir cells are in intimate contact with FSH-ir-DLK1-negative cells. In Dlk1 knockout (ko) mice, we detected a significantly lower number of GH-ir cells, a reduction in the FSH and PRL immunostaining intensity, and a significant decrease in FSH mRNA expression as compared with wild-type (wt) mice. An increase in pituitary GH mRNA expression and serum leptin levels was also found. These findings provide evidence supporting several regulatory function of DLK1 in the pituitary gland.
R A Puertas-Avendaño; M J González-Gómez; M D Ruvira; M J Ruiz-Hidalgo; N Morales-Delgado; J Laborda; C Díaz; A R Bello
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-14
Journal Detail:
Title:  Journal of neuroendocrinology     Volume:  -     ISSN:  1365-2826     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8913461     Medline TA:  J Neuroendocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Journal of Neuroendocrinology © 2011 Blackwell Publishing.
Cell Biology Section. School of Biology. University of La Laguna.Tenerife, Spain. Department of Inorganic and Organic Chemistry and Biochemistry. Biochemistry and Molecular Biology Branch, School of Medicine /Regional Centre for Biomedical Research, University of Castilla-La Mancha. Albacete, Spain. Department of Medical Sciences, School of Medicine / Regional Centre for Biomedical Research/Institute for Research in Neurological Disabilities, University of Castilla-La Mancha. Albacete, Spain.
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