Document Detail


Role of nitric oxide in the natriuretic and diuretic responses in pregnant rats.
MedLine Citation:
PMID:  12876067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Normal pregnancy is characterized by sodium conservation and increase in plasma volume, yet the natriuretic response to acute saline volume expansion (VE) is intact in pregnant rats. Nitric oxide (NO) has been suggested to play a role in renal and cardiovascular adaptations to normal pregnancy. The objective of this study was to determine the role of NO in the natriuretic and diuretic responses to VE during pregnancy. Infusion of NG-monomethyl-l-arginine (l-NMMA) was used to inhibit NO synthesis. Nine groups of Sprague-Dawley (SD) rats were studied: nonpregnant (NP-VE, n = 7), midterm pregnant (MP-VE, n = 8), and late-term pregnant (LP-VE, n = 7) SD groups that underwent VE alone after a control period; NP-l-NMMA (n = 7), MP-l-NMMA (n = 8), and LP-l-NMMA (n = 7) SD groups that were infused with l-NMMA after a control period; and another three groups of SD rats (NP-VE-l-NMMA, n = 8; MP-VE-l-NMMA, n = 7; and LP-VE-l-NMMA, n = 12) that underwent simultaneous VE and l-NMMA infusion after a control period. The change in fractional excretion of sodium was 7.22 +/- 1.03% for NPVE, 9.89 +/- 1.85% for NP-l-NMMA, and 17.66 +/- 1.85% for NP-VE-l-NMMA (P < 0.05 vs. NP-VE and NP-l-NMMA); 6.61 +/- 1.07% for MP-VE, 7.99 +/- 1.92% for MP-l-NMMA, and 10.24 +/- 1.91% for MP-VE-l-NMMA [not significant (NS) vs. MP-VE and MP-l-NMMA]; 8.20 +/- 1.92% for LP-VE, 8.09 +/- 0.70% for LP-l-NMMA, and 7.57 +/- 1.11% for LP-VE-l-NMMA (both NS vs. LP-VE and LP-l-NMMA). The increase in renal interstitial hydrostatic pressure was significantly greater in all NP compared with pregnant groups with similar experimental intervention (i.e., VE, l-NMMA, or VE-l-NMMA). In conclusion, the natriuretic and diuretic responses to VE and l-NMMA infusion were additive in NP but not in pregnant rats, indicating a possible lower ability of pregnant rats to respond to combined significant natriuretic and diuretic stimuli.
Authors:
Ali A Khraibi; Tianzheng Yu; Daiyi Tang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-07-22
Journal Detail:
Title:  American journal of physiology. Renal physiology     Volume:  285     ISSN:  1931-857X     ISO Abbreviation:  Am. J. Physiol. Renal Physiol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-08     Completed Date:  2003-12-02     Revised Date:  2011-04-28    
Medline Journal Info:
Nlm Unique ID:  100901990     Medline TA:  Am J Physiol Renal Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  F938-44     Citation Subset:  IM    
Affiliation:
Department of Physiological Sciences, Eastern Virginia Medical School, 700 Olney Rd., Norfolk, VA 23507-1696, USA. khraibaa@evms.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / drug effects
Diuresis / physiology*
Drug Combinations
Enzyme Inhibitors / administration & dosage,  pharmacology
Female
Hydrostatic Pressure
Infusions, Intravenous
Kidney / physiology
Natriuresis / physiology*
Nitric Oxide / antagonists & inhibitors,  physiology*
Pregnancy
Pregnancy, Animal / physiology*
Rats
Rats, Sprague-Dawley
Sodium Chloride / administration & dosage,  pharmacology
omega-N-Methylarginine / administration & dosage,  pharmacology
Grant Support
ID/Acronym/Agency:
HD-38240/HD/NICHD NIH HHS; HL-52765/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Drug Combinations; 0/Enzyme Inhibitors; 10102-43-9/Nitric Oxide; 17035-90-4/omega-N-Methylarginine; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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