Document Detail

Role of nitric oxide in gut ischemia-reperfusion-induced hepatic microvascular dysfunction.
MedLine Citation:
PMID:  9374696     Owner:  NLM     Status:  MEDLINE    
The overall objective of this study was to assess the contribution of an altered bioavailability of nitric oxide (NO) to the leukocyte adhesion and hypoxic stress elicited in the liver by gut ischemia-reperfusion (I/R). The accumulation of leukocytes, number of nonperfused sinusoids (NPS), and NADH autofluorescence were monitored (by intravital microscopy) in mouse liver after 15 min of superior mesenteric artery occlusion and 60 min of reperfusion. Leukostasis, NPS, and NADH autofluorescence (indicating hypoxia) were all increased in the liver at 60 min after gut I/R. The NO synthase inhibitor NG-monomethyl-L-arginine (L-NMMA) exaggerated the liver leukostasis elicited by gut I/R, responses that were prevented by coadministration of L-arginine. The NO donor diethylenetriamine-NO (DETA-NO) and L-arginine were both effective in attenuating the gut I/R-induced leukostasis and increased NADH autofluorescence, whereas neither DETA nor D-arginine exerted a protective action. These findings indicate that NO is an important determinant of the liver leukostasis, impaired sinusoidal perfusion, and tissue hypoxia elicited by gut I/R.
Y Horie; R Wolf; D N Granger
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  273     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-17     Completed Date:  1997-12-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G1007-13     Citation Subset:  IM    
Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport 71130, USA.
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MeSH Terms
Alanine Transaminase / blood
Arginine / pharmacology
Biological Availability
Intestines / blood supply*
Ischemia / physiopathology*
Leukocytes / drug effects,  physiology
Liver Circulation / drug effects,  physiology*
Mesenteric Arteries / physiology
Mesenteric Vascular Occlusion
Mice, Inbred C57BL
Microcirculation / drug effects,  physiology*
NAD / metabolism
Nitric Oxide / physiology*
Nitric Oxide Synthase / antagonists & inhibitors
Triazenes / pharmacology
omega-N-Methylarginine / pharmacology
Grant Support
Reg. No./Substance:
0/1-hydroxy-2-oxo-3,3-bis(2-aminoethyl)-1-triazene; 0/Triazenes; 10102-43-9/Nitric Oxide; 17035-90-4/omega-N-Methylarginine; 53-84-9/NAD; 74-79-3/Arginine; EC Oxide Synthase; EC Transaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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