Document Detail

Role of neutrophils in lung vascular injury and edema after premature birth in lambs.
MedLine Citation:
PMID:  9338441     Owner:  NLM     Status:  MEDLINE    
To investigate the role of neutrophils in the pathogenesis of respiratory distress after premature birth, we assessed the relationship between circulating neutrophil concentration and neutrophil accumulation in the lung, lung lymph and pleural liquid flow, and extravascular lung water in 10 chronically catheterized preterm lambs (127 +/- 1 days gestation) that were mechanically ventilated for 8 h after birth. Circulating neutrophil concentration transiently decreased within 2 h after birth and then returned to prenatal values by 6-8 h. The decrease in circulating neutrophil concentration was related directly to the accumulation of neutrophils in the air spaces, drainage of liquid and protein from the lung 6-8 h after delivery, and postmortem extravascular lung water. In additional studies, we intravenously administered mechlorethamine to 5 fetal lambs to reduce circulating neutrophils before delivery (neutrophil concentration before birth: 9 +/- 11 cells/microl). Compared with control lambs, neutrophil-depleted lambs had significantly less drainage of liquid (7.8 +/- 5.9 vs. 2.6 +/- 1.9 ml/h, respectively) and protein (116 +/- 74 vs. 42 +/- 27 mg/h, respectively) from the lung 6-8 h after birth and significantly less extravascular lung water at postmortem (6.5 +/- 0. 8 vs. 4.8 0.6 g/g dry lung, respectively). Thus neutrophils contribute to the pathogenesis of respiratory distress after premature birth by increasing lung vascular protein permeability and promoting lung edema.
D P Carlton; K H Albertine; S C Cho; M Lont; R D Bland
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  83     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-11-26     Completed Date:  1997-11-26     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1307-17     Citation Subset:  IM    
Department of Pediatrics, University of Utah, Salt Lake City, Utah 84132, USA.
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MeSH Terms
Alkylating Agents / pharmacology
Animals, Newborn / physiology*
Blood Gas Analysis
Extravascular Lung Water / physiology
Leukocyte Count
Lung / metabolism
Lymph / metabolism
Mechlorethamine / pharmacology
Neutrophils / drug effects,  physiology*
Proteins / metabolism
Pulmonary Circulation / physiology*
Pulmonary Edema / metabolism,  pathology*
Vascular Diseases / pathology*
Grant Support
Reg. No./Substance:
0/Alkylating Agents; 0/Proteins; 51-75-2/Mechlorethamine

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