Document Detail

Role of monocyte-lineage cells in prostate cancer cell invasion and tissue factor expression.
MedLine Citation:
PMID:  20607747     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Tissue factor (TF) is a cell surface glycoprotein intricately related to blood coagulation and inflammation. This study was performed to investigate the role of monocyte-lineage cells in prostate cancer cell TF expression and cell invasion. METHODS: Prostate cancer cell invasion was tested with and without added peripheral blood monocytes or human monocyte-lineage cell lines. TF neutralizing antibodies were used to determine the TF requirement for prostate cancer cell invasion activity. Immunohistochemistry was performed to identify prostate tissue CD68 positive monocyte-derived cells and prostate epithelial TF expression. RESULTS: Co-culture of PC-3, DU145, and LNCaP cells with isolated human monocytes significantly stimulated prostate cancer cell invasion activity. TF expression was greater in highly invasive prostate cancer cells and was induced in PC-3, DU145, and LNCaP cells by co-culture with U-937 cells, but not with THP-1 cells. TF neutralizing antibodies inhibited PC-3 cell invasion in co-cultures with monocyte-lineage U-937 or THP-1 cells. Prostate cancer tissues contained more CD68 positive cells in the stroma and epithelium (145 ± 53/mm(2)) than benign prostate (108 ± 31/mm(2)). Samples from advanced stage prostate cancer tended to contain more CD68 positive cells when compared with lower stage lesions. Prostatic adenocarcinoma demonstrated significantly increased TF expression compared with benign prostatic epithelium. CONCLUSIONS: This study shows that co-culture with monocyte-lineage cells induced prostate cancer cell invasion activity. PC-3 invasion and TF expression was induced in co-culture with U-937 cells and partially inhibited with TF neutralizing antibodies.
Paul F Lindholm; Yi Lu; Brian P Adley; Tudor Vladislav; Borko Jovanovic; Neela Sivapurapu; Ximing J Yang; André Kajdacsy-Balla
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Prostate     Volume:  70     ISSN:  1097-0045     ISO Abbreviation:  Prostate     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-29     Completed Date:  2010-10-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8101368     Medline TA:  Prostate     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1672-82     Citation Subset:  IM    
Department of Pathology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
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MeSH Terms
Adenocarcinoma / metabolism*,  pathology
Cell Growth Processes / physiology
Cell Line, Tumor
Cell Movement / physiology*
Coculture Techniques
Monocytes / metabolism,  pathology*
Prostatic Neoplasms / metabolism*,  pathology
Receptors, Complement / metabolism
Statistics, Nonparametric
Thromboplastin / biosynthesis*
Tissue Array Analysis
Grant Support
Reg. No./Substance:
0/C5AR1 protein, human; 0/Receptors, Complement; 9035-58-9/Thromboplastin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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