Document Detail


Role and modulation of CD16 expression on eosinophils by cytokines and immune complexes.
MedLine Citation:
PMID:  15153797     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Blood eosinophils express CD16 on their surface when stimulated in vitro with platelet-activating factor or IFNgamma. Transient expression of CD16 is also observed in vivo following aeroallergen challenge of asthmatic subjects. The present work is aimed at evaluating the possible mechanisms modulating eosinophil expression of CD16 and the biological functions of this receptor. METHODS: First, purified blood eosinophils were incubated with IL-1beta, IL-2, IL-4, IL-5, IL-9 or IL-16, GM-CSF, IFNgamma, eotaxin or 5-oxo-ETE and CD16 expression was measured. Second, the capacity of CD16 to mediate degranulation induced by IgG immune complexes (IC) was evaluated in eosinophils with low and high CD16 expression. Finally, serum allergen-specific IgE and IgG, and total IgE levels were measured at baseline in allergic asthmatics and correlated with changes observed in blood eosinophil CD16 expression (DeltaCD16) following allergen challenge. RESULTS: Only IFNgamma and IL-2 significantly increased the number of CD16+ eosinophils, respectively, 37 +/- 10% (p = 0.0038) and 38 +/- 8% (p = 0.0006), compared to control, 7 +/- 2%. IgG IC induced degranulation in eosinophils with low and high CD16 expression and monoclonal anti-CD16 and anti-CD32 antibodies inhibited this. IgG IC increased eosinophil CD16 expression (14 +/- 6%, p = 0.0008) and this effect was blocked by pretreatment with anti-CD32 antibodies. DeltaCD16 following allergen challenge correlated with the specific IgG/total IgE ratio (r(2) = 0.41, p = 0.036). CONCLUSION: These data suggest that formation of IgG IC is associated with surface eosinophil CD16 expression in asthma and that CD16 in cooperation with CD32 mediates IC-induced degranulation.
Authors:
Francis Davoine; Isabelle Labonté; Claudine Ferland; Bruce Mazer; Jamila Chakir; Michel Laviolette
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-05-19
Journal Detail:
Title:  International archives of allergy and immunology     Volume:  134     ISSN:  1018-2438     ISO Abbreviation:  Int. Arch. Allergy Immunol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-04     Completed Date:  2004-08-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9211652     Medline TA:  Int Arch Allergy Immunol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  165-72     Citation Subset:  IM    
Copyright Information:
Copyright 2004 S. Karger AG, Basel
Affiliation:
Unité de recherche en pneumologie, Centre de recherche de l'Hôpital Laval, Institut universitaire de cardiologie et pneumologie, Université Laval, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Antibody Specificity / immunology
Antigen-Antibody Complex / metabolism,  pharmacology*
Antigenic Modulation / physiology*
Antigens, Dermatophagoides / adverse effects,  pharmacology
Asthma / immunology,  metabolism
Biological Markers / blood
Bronchial Provocation Tests
Cell Degranulation / drug effects
Cytokines / metabolism,  pharmacology*
Eosinophils / cytology,  metabolism*
Humans
Immunoglobulin E / blood,  immunology
Immunoglobulin G / blood,  immunology
Interferon-gamma / metabolism,  pharmacology
Interleukins / metabolism,  pharmacology
Poaceae / adverse effects
Receptors, IgG / biosynthesis*
Statistics as Topic
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Antigens, Dermatophagoides; 0/Biological Markers; 0/Cytokines; 0/Immunoglobulin G; 0/Interleukins; 0/Receptors, IgG; 37341-29-0/Immunoglobulin E; 82115-62-6/Interferon-gamma

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