Document Detail

Role of mitochondria and mitochondrial cytochrome c in tubeimoside I-mediated apoptosis of human cervical carcinoma HeLa cell line.
MedLine Citation:
PMID:  16172906     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Tubeimoside I (TBMS1), a triterpenoid saponin, isolated from the tubers of Bolbostemma paniculatum, showed potent antitumor and antitumor-promoting effects. The objective of this study is to investigate the role of mitochondria and mitochondria cytochrome c in TBMS1-mediated apoptosis of human cervical carcinoma HeLa cell line. METHODS: Viability of HeLa cells was measured by MTT assay. Apoptotic induction by TBMS1 was determined by fluorescence microscopy, flow cytometry and gel electrophoresis of fragmented DNA. Mitochondrial transmembrane potential (Deltapsim) was assayed by flow cytometry. Cytochrome c (Cyt c) was detected by Western blotting. RESULTS: The results showed that Cyclosporin A (CsA) partly protected HeLa cells from growth inhibitory effect of TBMS1, and partly countered the ability of TBMS1 to rapidly induce apoptosis in HeLa cells, and that TBMS1 decreased Deltapsim and induced Cyt c release by a mechanism inhibited by CsA, and that TBMS1 induced apoptosis of HeLa cells dose-dependently in accordance with increase of cytosolic Cyt c. CONCLUSIONS: TBMS1 opens the permeability transition (PT) pore, thereby decreasing Deltapsim, releasing Cyt c from mitochondria, and further causing a series of events consistent with established mechanistic models of apoptosis.
Fang Wang; Rundi Ma; Lijian Yu
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2005-09-20
Journal Detail:
Title:  Cancer chemotherapy and pharmacology     Volume:  57     ISSN:  0344-5704     ISO Abbreviation:  Cancer Chemother. Pharmacol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2005-12-23     Completed Date:  2006-02-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806519     Medline TA:  Cancer Chemother Pharmacol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  389-99     Citation Subset:  IM    
Guangdong Provincial Key Laboratory of Marine Materia Medica, Zhanjiang Ocean University, Guangdong, People's Republic of China.
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MeSH Terms
Acridine Orange / diagnostic use
Annexin A5 / diagnostic use
Apoptosis / drug effects*,  physiology
Blotting, Western
Cell Membrane / chemistry,  drug effects
Cell Nucleus / drug effects
Cell Proliferation / drug effects
Chromatin Assembly and Disassembly / drug effects
Cucurbitaceae / chemistry
Cyclosporine / pharmacology
Cytochromes c / physiology*,  secretion
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Drugs, Chinese Herbal / chemistry,  pharmacology
Ethidium / diagnostic use
Hela Cells
Microscopy, Electron
Microscopy, Fluorescence / methods
Mitochondria / drug effects,  physiology*,  ultrastructure
Molecular Structure
Propidium / diagnostic use
Saponins / chemistry,  pharmacology*
Triterpenes / chemistry,  pharmacology*
Reg. No./Substance:
0/Annexin A5; 0/Drugs, Chinese Herbal; 0/Saponins; 0/Triterpenes; 102040-03-9/tubeimoside I; 3546-21-2/Ethidium; 36015-30-2/Propidium; 59865-13-3/Cyclosporine; 65-61-2/Acridine Orange; 9007-43-6/Cytochromes c

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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