Document Detail


Role of mineralocorticoid receptor on experimental cerebral aneurysms in rats.
MedLine Citation:
PMID:  19620512     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Activation of the renin-angiotensin (Ang)-aldosterone system is involved in the pathology of vascular diseases. Although the blockade of the mineralocorticoid receptor protects against vascular diseases, its role in cerebral aneurysms remains to be elucidated. We treated female rats subjected to renal hypertension, increased hemodynamic stress, and estrogen deficiency for 3 months with the mineralocorticoid receptor blocker eplerenone (30 or 100 mg/kg per day) or vehicle (vehicle control). Eplerenone reduced the incidence of cerebral aneurysms and saline intake without lowering of the blood pressure. In the aneurysmal wall, the production of Ang II and nitrotyrosine was increased. The mRNA levels of Ang-converting enzyme 1 and NADPH oxidase subunits NOX4, Rac1, monocyte chemoattractant protein 1, and matrix metalloproteinase 9 were increased. Eplerenone brought about a reduction in these molecules, suggesting that mineralocorticoid receptor blockade suppresses cerebral aneurysm formation by inhibiting oxidative stress, inflammatory factors, local renin-Ang system activation, and saline intake. Other female rats implanted with pellets of the mineralocorticoid receptor agonist deoxycorticosterone acetate manifested a high incidence of cerebral aneurysm formation and the upregulation of molecules related to oxidative stress, inflammatory factors, and the local renin-Ang system; their saline intake was increased. We demonstrate that mineralocorticoid receptor activation at least partly contributes to the pathogenesis of cerebral aneurysms.
Authors:
Yoshiteru Tada; Keiko T Kitazato; Tetsuya Tamura; Kenji Yagi; Kenji Shimada; Tomoya Kinouchi; Junichiro Satomi; Shinji Nagahiro
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-20
Journal Detail:
Title:  Hypertension     Volume:  54     ISSN:  1524-4563     ISO Abbreviation:  Hypertension     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-20     Completed Date:  2009-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  United States    
Other Details:
Languages:  eng     Pagination:  552-7     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, Japan. yoshiteru.tada@hotmail.co.jp.
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MeSH Terms
Descriptor/Qualifier:
Aldosterone / blood
Aldosterone Antagonists / pharmacology
Angiotensin II / metabolism
Animals
Blood Pressure / drug effects
Cerebral Arteries / drug effects,  metabolism,  pathology
Chemokine CCL2 / genetics,  metabolism
Female
Gene Expression / drug effects
Hypertension, Renal / drug therapy,  physiopathology
Immunohistochemistry
Intracranial Aneurysm / blood,  physiopathology,  prevention & control*
NADPH Oxidase / genetics,  metabolism
Ovariectomy
Oxidative Stress / drug effects
Peptidyl-Dipeptidase A / genetics,  metabolism
RNA, Messenger / genetics,  metabolism
Rats
Rats, Sprague-Dawley
Receptors, Mineralocorticoid / antagonists & inhibitors*,  physiology
Renin-Angiotensin System / drug effects
Reverse Transcriptase Polymerase Chain Reaction
Spironolactone / analogs & derivatives*,  pharmacology
Tyrosine / analogs & derivatives,  metabolism
Chemical
Reg. No./Substance:
0/Aldosterone Antagonists; 0/Ccl2 protein, rat; 0/Chemokine CCL2; 0/RNA, Messenger; 0/Receptors, Mineralocorticoid; 0/eplerenone; 11128-99-7/Angiotensin II; 3604-79-3/3-nitrotyrosine; 52-01-7/Spironolactone; 52-39-1/Aldosterone; 55520-40-6/Tyrosine; EC 1.6.3.1/NADPH Oxidase; EC 3.4.15.1/Peptidyl-Dipeptidase A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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