Document Detail


Role of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12.
MedLine Citation:
PMID:  2443485     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mutation in the tolC locus greatly reduces normal synthesis of OmpF, a major porin protein of Escherichia coli K-12. Experiments that use ompF-ompC chimeric genes demonstrate that a tolC mutation exerts its effect at either the promoter or the amino-terminal end of the ompF gene. Direct analysis of ompF mRNA from tolC+ and tolC strains showed that the amount of ompF transcript in the latter was greatly reduced. We have also observed that, in addition to reducing the amount of OmpF, a tolC mutation increases the level of OmpC protein to a much greater extent than occurs in an OmpF mutant and also increases micF RNA synthesis as shown by increased beta-galactosidase synthesis in a micF-lacZ fusion strain. Based on these observations, we suggest that an increased expression of the micF gene in a tolC mutant results in the reduced expression of ompF and that a major effect of the tolC mutation may be to push the porin-regulating system to favor ompC and micF to a greater extent than under high-osmolarity conditions.
Authors:
R Misra; P R Reeves
Related Documents :
15484955 - Effect of dietary restriction, pregnancy, and fetal type on intestinal cellularity and ...
19436635 - Acephalous lamb from an in vitro-produced sheep embryo.
11263815 - Oxytocin-induced cervical dilation and cervical manipulation in sheep: effects on lapar...
23199145 - Biomarker development for non-invasive prenatal diagnosis of fetal aneuploidies: predic...
10473895 - Influence of maternal age and duration of pregnancy on serum concentrations of polychlo...
24873115 - The role of yoga: breathing, meditation and optimal fetal positioning.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  169     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1987-11-05     Completed Date:  1987-11-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4722-30     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Sydney, New South Wales, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Outer Membrane Proteins / biosynthesis,  genetics*
Cloning, Molecular
Culture Media
DNA Restriction Enzymes
DNA, Bacterial / analysis
Electrophoresis, Agar Gel
Escherichia coli / genetics*,  metabolism
Gene Expression Regulation*
Genes, Bacterial*
Genes, Regulator
Mutation
Nucleic Acid Hybridization
Osmolar Concentration
Plasmids
RNA, Bacterial / analysis
RNA, Messenger / analysis
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Culture Media; 0/DNA, Bacterial; 0/RNA, Bacterial; 0/RNA, Messenger; EC 3.1.21.-/DNA Restriction Enzymes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Regulation and initiation of cenB transcripts of Cellulomonas fimi.
Next Document:  Replication control genes of plasmid pE194.