Document Detail

Role of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12.
MedLine Citation:
PMID:  2443485     Owner:  NLM     Status:  MEDLINE    
Mutation in the tolC locus greatly reduces normal synthesis of OmpF, a major porin protein of Escherichia coli K-12. Experiments that use ompF-ompC chimeric genes demonstrate that a tolC mutation exerts its effect at either the promoter or the amino-terminal end of the ompF gene. Direct analysis of ompF mRNA from tolC+ and tolC strains showed that the amount of ompF transcript in the latter was greatly reduced. We have also observed that, in addition to reducing the amount of OmpF, a tolC mutation increases the level of OmpC protein to a much greater extent than occurs in an OmpF mutant and also increases micF RNA synthesis as shown by increased beta-galactosidase synthesis in a micF-lacZ fusion strain. Based on these observations, we suggest that an increased expression of the micF gene in a tolC mutant results in the reduced expression of ompF and that a major effect of the tolC mutation may be to push the porin-regulating system to favor ompC and micF to a greater extent than under high-osmolarity conditions.
R Misra; P R Reeves
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  169     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1987-11-05     Completed Date:  1987-11-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4722-30     Citation Subset:  IM    
Department of Microbiology, University of Sydney, New South Wales, Australia.
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MeSH Terms
Bacterial Outer Membrane Proteins / biosynthesis,  genetics*
Cloning, Molecular
Culture Media
DNA Restriction Enzymes
DNA, Bacterial / analysis
Electrophoresis, Agar Gel
Escherichia coli / genetics*,  metabolism
Gene Expression Regulation*
Genes, Bacterial*
Genes, Regulator
Nucleic Acid Hybridization
Osmolar Concentration
RNA, Bacterial / analysis
RNA, Messenger / analysis
Transcription, Genetic
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Culture Media; 0/DNA, Bacterial; 0/RNA, Bacterial; 0/RNA, Messenger; EC 3.1.21.-/DNA Restriction Enzymes

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