| Role of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12. | |
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MedLine Citation:
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PMID: 2443485 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mutation in the tolC locus greatly reduces normal synthesis of OmpF, a major porin protein of Escherichia coli K-12. Experiments that use ompF-ompC chimeric genes demonstrate that a tolC mutation exerts its effect at either the promoter or the amino-terminal end of the ompF gene. Direct analysis of ompF mRNA from tolC+ and tolC strains showed that the amount of ompF transcript in the latter was greatly reduced. We have also observed that, in addition to reducing the amount of OmpF, a tolC mutation increases the level of OmpC protein to a much greater extent than occurs in an OmpF mutant and also increases micF RNA synthesis as shown by increased beta-galactosidase synthesis in a micF-lacZ fusion strain. Based on these observations, we suggest that an increased expression of the micF gene in a tolC mutant results in the reduced expression of ompF and that a major effect of the tolC mutation may be to push the porin-regulating system to favor ompC and micF to a greater extent than under high-osmolarity conditions. |
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Authors:
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R Misra; P R Reeves |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 169 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1987 Oct |
Date Detail:
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Created Date: 1987-11-05 Completed Date: 1987-11-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4722-30 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Sydney, New South Wales, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Outer Membrane Proteins
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biosynthesis,
genetics* Cloning, Molecular Culture Media DNA Restriction Enzymes DNA, Bacterial / analysis Electrophoresis, Agar Gel Escherichia coli / genetics*, metabolism Gene Expression Regulation* Genes, Bacterial* Genes, Regulator Mutation Nucleic Acid Hybridization Osmolar Concentration Plasmids RNA, Bacterial / analysis RNA, Messenger / analysis Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins; 0/Culture Media; 0/DNA, Bacterial; 0/RNA, Bacterial; 0/RNA, Messenger; EC 3.1.21.-/DNA Restriction Enzymes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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