Document Detail

Role of metalloprotease disintegrin ADAM12 in determination of quiescent reserve cells during myogenic differentiation in vitro.
MedLine Citation:
PMID:  12972593     Owner:  NLM     Status:  MEDLINE    
Skeletal myoblasts grown in vitro and induced to differentiate either form differentiated multinucleated myotubes or give rise to quiescent, undifferentiated "reserve cells" that share several characteristics with muscle satellite cells. The mechanism of determination of reserve cells is poorly understood. We find that the expression level of the metalloprotease disintegrin ADAM12 is much higher in proliferating C2C12 myoblasts and in reserve cells than in myotubes. Inhibition of ADAM12 expression in differentiating C2C12 cultures by small interfering RNA is accompanied by lower expression levels of both quiescence markers (retinoblastoma-related protein p130 and cell cycle inhibitor p27) and differentiation markers (myogenin and integrin alpha7A isoform). Overexpression of ADAM12 in C2C12 cells under conditions that promote cell cycle progression leads to upregulation of p130 and p27, cell cycle arrest, and downregulation of MyoD. Thus, enhanced expression of ADAM12 induces a quiescence-like phenotype and does not stimulate differentiation. We also show that the region extending from the disintegrin to the transmembrane domain of ADAM12 and containing cell adhesion activity as well as the cytoplasmic domain of ADAM12 are required for ADAM12-mediated cell cycle arrest, while the metalloprotease domain is not essential. Our results suggest that ADAM12-mediated adhesion and/or signaling may play a role in determination of the pool of reserve cells during myoblast differentiation.
Yi Cao; Zhefeng Zhao; Joanna Gruszczynska-Biegala; Anna Zolkiewska
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  23     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-15     Completed Date:  2003-11-04     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6725-38     Citation Subset:  IM    
Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA.
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MeSH Terms
ADAM Proteins
Biological Markers
Carrier Proteins / metabolism
Cell Cycle
Cell Differentiation / physiology
Cell Division
Cell Line
Disintegrins / metabolism*
Gene Expression Regulation
Integrin alpha Chains / metabolism
Membrane Proteins / chemistry,  genetics,  physiology*
Metalloendopeptidases / metabolism*
Muscle Fibers, Skeletal / physiology
Muscle Proteins / chemistry,  genetics,  physiology*
Muscle, Skeletal / embryology*
MyoD Protein / metabolism
Myogenin / metabolism
Protein Structure, Tertiary
RNA, Small Interfering / metabolism
Retinoblastoma-Binding Protein 1
Grant Support
Reg. No./Substance:
0/Arid4a protein, mouse; 0/Biological Markers; 0/Carrier Proteins; 0/Disintegrins; 0/Integrin alpha Chains; 0/Membrane Proteins; 0/Muscle Proteins; 0/MyoD Protein; 0/Myog protein, mouse; 0/Myogenin; 0/RNA, Small Interfering; 0/Retinoblastoma-Binding Protein 1; EC 3.4.24.-/ADAM Proteins; EC 3.4.24.-/Adam12 protein, mouse; EC 3.4.24.-/Metalloendopeptidases

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