Document Detail


Role of membrane transport in interorgan amino acid flow between muscle and small intestine.
MedLine Citation:
PMID:  7783655     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the fasting state, amino acids are released from the periphery to be used in splanchnic tissues. To understand the mechanism of such interorgan substrate exchange at the tissue level, we have determined the relationships between inward and outward amino acid transport and intracellular amino acid kinetics in the small intestine and skeletal muscle of postabsorptive anesthetized dogs. In the gut, amino acids appearing intracellularly (from inward transport, protein degradation, and absorption from the lumen) were used for protein synthesis more efficiently (P < .05) than in muscle (phenylalanine, 55% +/- 5% v 13% +/- 3%; lysine, 70% +/- 7% v 28% +/- 3%). In contrast, in muscle, amino acids appearing intracellularly (from inward transport and protein degradation) were preferentially (P < .05) released into the bloodstream, as opposed to being incorporated into protein (phenylalanine, 87% +/- 4%; lysine, 72% +/- 3%). Inward transport accounted for a greater (P < .05) proportion of total intracellular amino acid appearance in the gut than in muscle (leucine, 63% +/- 3% v 37 +/- 3%; valine, 75% +/- 5% v 53% +/- 3%; phenylalanine, 66% +/- 1% v 50% +/- 4%; lysine, 52% +/- 2% v 31% +/- 2%). We conclude that differences in transmembrane amino acid transport kinetics in both the inward and outward directions contribute to the net flow of amino acids from the muscle to the gut in the fasting state.
Authors:
G Biolo; X J Zhang; R R Wolfe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Metabolism: clinical and experimental     Volume:  44     ISSN:  0026-0495     ISO Abbreviation:  Metab. Clin. Exp.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-07-14     Completed Date:  1995-07-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375267     Medline TA:  Metabolism     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  719-24     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, University of Texas Medical Branch, Galveston, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / blood,  metabolism*
Animals
Arteries
Biological Transport
Dogs
Female
Intestine, Small / metabolism*
Male
Models, Biological
Muscle Proteins / metabolism
Muscle, Skeletal / metabolism*
Veins
Grant Support
ID/Acronym/Agency:
00073//PHS HHS; DK 38010-05/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Muscle Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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