| Role of membrane lipids in the immunological killing of tumor cells: II. Effector cell lipids. | |
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MedLine Citation:
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PMID: 6621258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peritoneal macrophages (M phi) from mice become cytotoxic after incubation in lymphokine (LK)-rich supernatants of antigen-stimulated spleen cell cultures. Tumoricidal activity is evident with M phi treated with LK for 4 hr, becomes maximal after 8-12 hr incubation and decreases to control levels by 24-36 hr. To gain insight into LK-induced functional changes, the lipid composition of M phi cultured with LK for 0-36 hr was analyzed by high pressure liquid chromatography. LK induced marked changes in M phi lipid composition: cellular content of cholesterol (CHOL) and polyunsaturated fatty acids increased 2- to 3-fold after 8 hr when the cells showed maximal tumoricidal activity. Cellular lipid and fatty acid content returned to control levels by 24 hr when the M phi had lost tumoricidal activity. These changes were not observed with equal numbers of M phi cultured in control supernatants. To analyze further the role of CHOL and unsaturated fatty acids in M phi tumor cytotoxicity, M phi were enriched in CHOL or linolenic acid (18:3) and tested for their ability to kill 1023 tumor cells. Within 1 hr of culture, M phi showed a 3- to 4-fold increase in CHOL or 18:3 content. 18:3-enriched cells were markedly tumoricidal, whereas controls cultured in delipidized medium alone or enriched with saturated fatty acids were cytotoxic. CHOL-enriched M phi were not tumoricidal; indeed, these cells were inhibited in their killing after treatment with LK compared to M phi cultured in delipidized medium with LK alone. These results suggest that UFA aids, whereas CHOL negates, expression of M phi tumor cytotoxicity. |
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Authors:
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S I Schlager; M S Meltzer; L D Madden |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Lipids Volume: 18 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1983 Jul |
Date Detail:
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Created Date: 1983-11-23 Completed Date: 1983-11-23 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 483-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane / immunology Cytotoxicity, Immunologic* / drug effects Fibrosarcoma / immunology* Lipid Metabolism* Lymphokines / immunology Macrophages / drug effects, immunology*, metabolism Membrane Lipids / immunology* Mice Mice, Inbred Strains Sarcoma, Experimental / immunology |
| Chemical | |
Reg. No./Substance:
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0/Lymphokines; 0/Membrane Lipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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