| Role of medial smooth muscle cell function in antithrombogenicity of vein grafts. | |
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MedLine Citation:
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PMID: 9610349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To determine the significance of prostaglandin I2 (PGI2) production by medial smooth muscle cells (SMCs), we assessed PGI2 production from denuded vein grafts and their antithrombogenicity. MATERIALS AND METHODS: A total of 30 dogs were divided into two groups: in group I, 14 dogs (27 veins, 188 segments) were used to assay PGI2 production, which was measured as 6-keto-PGF1 alpha. In group II, 16 dogs were used for an implantation study and the thrombus-free surface (TFS) score was calculated 24 h after implantation. Both groups contained the following subgroups: subgroup A, freshly harvested veins; subgroup B, endothelial denuded veins; subgroup C, veins frozen and cryopreserved; subgroup D, veins treated with protease. RESULTS: Values of 6-keto-PGF1 alpha (pg/mg/min) for basal and stimulated states in subgroup I-A through I-D were 58.0 +/- 8.9 and 530.6 +/- 74.7, 26.3 +/- 4.7 and 82.3 +/- 11.4, 17.9 +/- 1.3 and 39.4 +/- 3.3, and 13.3 +/- 1.8 and 32.2 +/- 6.2, respectively. The PGI2 production in subgroup I-A were significantly higher than those in the other three subgroups (p < 0.01 for basal and stimulated), while the production in subgroup I-B was also significantly higher than those in the remaining two (p < 0.05 for basal and stimulated). The TFS scores in subgroup II-A through II-D were 98 +/- 2%, 90 +/- 2%, 38 +/- 5%, and 15 +/- 7%, respectively, showing significantly superior antithrombogenicity in subgroup II-B, as well as in II-A, when compared with the remaining two (p = 0.014). CONCLUSION: The amount of PGI2 generated by the medial SMCs may be sufficient for maintaining the antithrombogenicity of the endothelial denuded AVGs. |
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Authors:
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E Yatsuyanagi; T Sasajima; K Goh; M Inaba; Y Kubo |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery Volume: 15 ISSN: 1078-5884 ISO Abbreviation: Eur J Vasc Endovasc Surg Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1998-06-10 Completed Date: 1998-06-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9512728 Medline TA: Eur J Vasc Endovasc Surg Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 350-6 Citation Subset: IM |
Affiliation:
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First Department of Surgery, Asahikawa Medical College, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Abdominal / surgery Dogs Epoprostenol / biosynthesis* Female Fibrinolysis Male Microscopy, Electron, Scanning Muscle, Smooth, Vascular / metabolism*, ultrastructure Radioimmunoassay Random Allocation Reference Values Transplantation, Autologous Veins / metabolism*, transplantation* |
| Chemical | |
Reg. No./Substance:
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35121-78-9/Epoprostenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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