Document Detail

Role of medial smooth muscle cell function in antithrombogenicity of vein grafts.
MedLine Citation:
PMID:  9610349     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To determine the significance of prostaglandin I2 (PGI2) production by medial smooth muscle cells (SMCs), we assessed PGI2 production from denuded vein grafts and their antithrombogenicity. MATERIALS AND METHODS: A total of 30 dogs were divided into two groups: in group I, 14 dogs (27 veins, 188 segments) were used to assay PGI2 production, which was measured as 6-keto-PGF1 alpha. In group II, 16 dogs were used for an implantation study and the thrombus-free surface (TFS) score was calculated 24 h after implantation. Both groups contained the following subgroups: subgroup A, freshly harvested veins; subgroup B, endothelial denuded veins; subgroup C, veins frozen and cryopreserved; subgroup D, veins treated with protease. RESULTS: Values of 6-keto-PGF1 alpha (pg/mg/min) for basal and stimulated states in subgroup I-A through I-D were 58.0 +/- 8.9 and 530.6 +/- 74.7, 26.3 +/- 4.7 and 82.3 +/- 11.4, 17.9 +/- 1.3 and 39.4 +/- 3.3, and 13.3 +/- 1.8 and 32.2 +/- 6.2, respectively. The PGI2 production in subgroup I-A were significantly higher than those in the other three subgroups (p < 0.01 for basal and stimulated), while the production in subgroup I-B was also significantly higher than those in the remaining two (p < 0.05 for basal and stimulated). The TFS scores in subgroup II-A through II-D were 98 +/- 2%, 90 +/- 2%, 38 +/- 5%, and 15 +/- 7%, respectively, showing significantly superior antithrombogenicity in subgroup II-B, as well as in II-A, when compared with the remaining two (p = 0.014). CONCLUSION: The amount of PGI2 generated by the medial SMCs may be sufficient for maintaining the antithrombogenicity of the endothelial denuded AVGs.
E Yatsuyanagi; T Sasajima; K Goh; M Inaba; Y Kubo
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery     Volume:  15     ISSN:  1078-5884     ISO Abbreviation:  Eur J Vasc Endovasc Surg     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-06-10     Completed Date:  1998-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9512728     Medline TA:  Eur J Vasc Endovasc Surg     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  350-6     Citation Subset:  IM    
First Department of Surgery, Asahikawa Medical College, Japan.
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MeSH Terms
Aorta, Abdominal / surgery
Epoprostenol / biosynthesis*
Microscopy, Electron, Scanning
Muscle, Smooth, Vascular / metabolism*,  ultrastructure
Random Allocation
Reference Values
Transplantation, Autologous
Veins / metabolism*,  transplantation*
Reg. No./Substance:

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