| Role for mannose-sensitive hemagglutinin in promoting interactions between Vibrio cholerae El Tor and mussel hemolymph. | |
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MedLine Citation:
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PMID: 12957968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The role of mannose-sensitive hemagglutinin (MSHA) in Vibrio cholerae O1 El Tor interactions with hemolymph of the mussel Mytilus galloprovincialis was studied. Bacterial adherence to and association with hemocytes were evaluated at 4 and 18 degrees C, respectively. In hemolymph serum, the wild-type strain N16961 adhered to and associated with hemocytes about twofold more efficiently than its mutant lacking MSHA. In artificial seawater (ASW), no significant differences between the two strains were observed. N16961 was also more sensitive to hemocyte bactericidal activity than its MSHA mutant; in fact, the percentages of killed bacteria after 120 min of incubation were 60 and 34%, respectively. The addition of D-mannose abolished the serum-mediated increase in adherence, association, and sensitivity to killing of the wild-type strain without affecting the interactions of the mutant. A similar increase in N16961 adherence to hemocytes was observed when serum was adsorbed with MSHA-deficient bacteria. In contrast, serum adsorbed with either wild-type V. cholerae El Tor or wild-type Escherichia coli carrying type 1 fimbriae was no longer able to increase adherence of N16961 to hemocytes. The results indicate that hemolymph-soluble factors are involved in interactions between hemocytes and mannose-sensitive adhesins. |
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Authors:
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Massimiliano Zampini; Laura Canesi; Michele Betti; Caterina Ciacci; Renato Tarsi; Gabriella Gallo; Carla Pruzzo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 69 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-09-05 Completed Date: 2004-01-12 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 5711-5 Citation Subset: IM |
Affiliation:
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Istituto di Microbiologia e Scienze Biomediche, Università Politecnica delle Marche, Ancona, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Proteins / isolation & purification, physiology* Bivalvia / microbiology* Fimbriae Proteins / isolation & purification, physiology* Fimbriae, Bacterial / physiology Hemagglutinins / isolation & purification, physiology* Hemocytes / microbiology Hemolymph / microbiology* Mannose-Binding Lectin Vibrio cholerae / isolation & purification, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Hemagglutinins; 0/Mannose-Binding Lectin; 0/MshA protein, Vibrio cholerae; 147680-16-8/Fimbriae Proteins |
| Comments/Corrections | |
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