|Role of mammalian Mre11 in classical and alternative nonhomologous end joining.|
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|PMID: 19633669 Owner: NLM Status: MEDLINE|
|The mammalian Mre11-Rad50-Nbs1 (MRN) complex coordinates double-strand break signaling with repair by homologous recombination and is associated with the H2A.X chromatin response to double-strand breaks, but its role in nonhomologous end joining (NHEJ) is less clear. Here we show that Mre11 promotes efficient NHEJ in both wild-type and Xrcc4(-/-) mouse embryonic stem cells. Depletion of Mre11 reduces the use of microhomology during NHEJ in Xrcc4(+/+) cells and suppresses end resection in Xrcc4(-/-) cells, revealing specific roles for Mre11 in both classical and alternative NHEJ. The NHEJ function of Mre11 is independent of H2A.X. We propose a model in which both enzymatic and scaffolding functions of Mre11 cooperate to support mammalian NHEJ.|
|Anyong Xie; Amy Kwok; Ralph Scully|
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|Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-07-26|
|Title: Nature structural & molecular biology Volume: 16 ISSN: 1545-9985 ISO Abbreviation: Nat. Struct. Mol. Biol. Publication Date: 2009 Aug|
|Created Date: 2009-08-05 Completed Date: 2009-09-16 Revised Date: 2014-09-17|
Medline Journal Info:
|Nlm Unique ID: 101186374 Medline TA: Nat Struct Mol Biol Country: United States|
|Languages: eng Pagination: 814-8 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Ataxia Telangiectasia Mutated Proteins
Cell Cycle Proteins / antagonists & inhibitors, genetics, metabolism
DNA Repair Enzymes / genetics, metabolism*
DNA-Binding Proteins / antagonists & inhibitors, genetics, metabolism*
Embryonic Stem Cells / cytology, metabolism, radiation effects
Green Fluorescent Proteins / genetics, metabolism
Histones / genetics, metabolism
Protein-Serine-Threonine Kinases / antagonists & inhibitors, genetics, metabolism
Tumor Suppressor Proteins / antagonists & inhibitors, genetics, metabolism
|GM073894/GM/NIGMS NIH HHS; R01 CA095175/CA/NCI NIH HHS; R01 CA095175-06A1/CA/NCI NIH HHS; R01 GM073894/GM/NIGMS NIH HHS; R01 GM073894-04/GM/NIGMS NIH HHS; R01S CA095175/CA/NCI NIH HHS|
|0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/H2AX protein, mouse; 0/Histones; 0/Mre11a protein, mouse; 0/Tumor Suppressor Proteins; 0/XRCC4 protein, mouse; 147336-22-9/Green Fluorescent Proteins; EC 126.96.36.199/Ataxia Telangiectasia Mutated Proteins; EC 188.8.131.52/Atm protein, mouse; EC 184.108.40.206/Protein-Serine-Threonine Kinases; EC 6.5.1.-/DNA Repair Enzymes|
|Nat Struct Mol Biol. 2009 Aug;16(8):798-800
Journal ID (nlm-journal-id): 101186374
Journal ID (pubmed-jr-id): 31761
Journal ID (nlm-ta): Nat Struct Mol Biol
nihms-submitted publication date: Day: 23 Month: 6 Year: 2009
Electronic publication date: Day: 26 Month: 7 Year: 2009
Print publication date: Month: 8 Year: 2009
pmc-release publication date: Day: 1 Month: 2 Year: 2010
Volume: 16 Issue: 8
First Page: 814 Last Page: 818
PubMed Id: 19633669
R01 GM073894-04 ||GM
R01 CA095175-06A1 ||CA
National Institute of General Medical Sciences : NIGMS
National Cancer Institute : NCI
|Role of mammalian Mre11 in classical and alternative non-homologous end joining|
|Department of Medicine, Harvard Medical School and Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215
|1 Corresponding authors: R.S. email@example.com. Telephone 617-735-2045. Fax 617-735-2222; A.X. firstname.lastname@example.org Telephone 617-735-2042. Fax 617-735-2222
A.X. designed and performed the experiments. R.S. participated in the design of the experiments. A.K. assisted on Southern blotting and DNA sequencing. A.X. and R.S. analyzed the data and wrote the manuscript.
DNA double strand breaks (DSBs) provoke a complex set of cellular responses, which, if defective, can perturb development or promote cancer in higher eukaryotes. The Mre11/Rad50/Nbs1 (MRN) complex in vertebrates and the homologous Mre11/Rad50/Xrs2 (MRX) complex in yeast act as DSB sensors and also support DSB repair 1. MRN binds to DNA ends, recruiting and activating the Atm signaling kinase 2,3. One of the targets of Atm is histone H2A.X, which is phosphorylated on serine 139 to form “γ-H2A.X”, recruiting Mdc1, MRN and other protein complexes to chromatin near the DSB 4. Thus, MRN exists in two fractions near the DSB – an H2A.X-independent fraction at the DSB and an H2A.X/Mdc1-dependent fraction on chromatin 5. Loss of Mre11, Rad50 or Nbs1 results in embryonic lethality in mice 6-8. Hypomorphic mutations in human MRE11 or NBS1 lead to ataxia telangiectasia-like disorder (ATLD) or Nijmegen breakage syndrome (NBS) respectively 9,10. Cells derived from individuals with either disorder show radiation hypersensitivity, chromosomal instability and DSB repair defects.
DSBs in eukaryotes are repaired primarily by homologous recombination (HR) or non-homologous end joining (NHEJ). In mammalian cells, the classical NHEJ pathway uses Xrcc4/DNA ligase 4 to catalyze ligation of DNA ends 11,12. A robust alternative, Xrcc4-independent NHEJ pathway has been identified 13-16. DSB repair products in Xrcc4−/− cells reveal frequent deletions associated with short tracts of homology at the repair junction, an outcome termed microhomology-mediated end joining (MMEJ) 17.
The MRN complex has been implicated in HR in both yeast and vertebrates. Mre11 collaborates with Sae2/Ctp1/CtIP in end processing for HR 18-21, and MRN/MRX may also promote HR by tethering DNA ends 22-24. In Saccharomyces cerevisiae, MRX facilitates NHEJ 25,26 and has also been implicated in MMEJ 27. Early efforts to demonstrate a role for vertebrate MRN in NHEJ led to contradictory conclusions 28-30. However, recent work has revealed a role for MRN in NHEJ during V(D)J recombination in developing immunocytes 31,32. To what extent mammalian MRN influences NHEJ of unscheduled DSBs is not clear.
To study the role of Mre11 in mammalian NHEJ, including Xrcc4-independent NHEJ, we generated isogenic Xrcc4+/+ and Xrcc4−/− mouse embryonic stem (ES) cells harboring a single copy of an NHEJ reporter, in which NHEJ is triggered in response to tandem site-specific chromosomal DSBs. Our work reveals a critical role for Mre11 in both Xrcc4-dependent and Xrcc4-independent NHEJ in mammalian cells and suggests that Mre11 participates in the processing of DSBs for MMEJ in cells lacking Xrcc4.
We developed NHEJ reporters in which translation of a wild-type copy of the gene encoding enhanced green fluorescent protein (“GFP”) is suppressed by an upstream, out-of-frame translation start site (“Koz-ATG”, Fig. 1a). Tandem DSBs introduced by the rare-cutting homing endonuclease, I-SceI, can release (“pop out”) Koz-ATG, and religation of the DNA ends allows translation of GFP in the correct frame. We introduced, in parallel, two NHEJ reporters – one that generates fully cohesive 4 nucleotide (nt) overhangs (sGEJ) (Fig. 1b) and the other containing partially cohesive 4 nt overhangs (vGEJ; Fig. 1c) – into Xrcc4flox/flox mouse ES cells, where Xrcc4 alleles can be conditionally deleted 33. We used Southern blotting to identify several clones that carry only one, intact, randomly integrated copy of the relevant NHEJ reporter. Background levels of GFP in each clone measured by fluorescence-activated cell sorting (FACS) were always < 0.01% (Supplementary Fig. 1). Expression of I-SceI, but not control empty vector, stimulated production of GFP in each cell type up to ∼40% in some reporter clones (Fig. 1b,c and Supplementary Fig 1). We used transient expression of the Cre recombinase to generate derivative clones of each reporter type that are either Xrcc4+/+, Xrcc4+/− or Xrcc4−/− (confirmed by Southern analysis). Homozygous deletion of Xrcc4 diminished the efficiency of NHEJ in all clones, but a robust residual end joining activity was noted in Xrcc4−/− cells (Fig. 1b,c). The emergence of GFP+ products of NHEJ was delayed in Xrcc4−/− cells compared to wild-type isogenic clones, implying that the kinetics of rejoining are slower in alternative NHEJ (Supplementary Fig. 2a,b). Expression of wild-type human XRCC4 in Xrcc4−/− cells complemented the NHEJ defect (Supplementary Fig. 2c).
To determine whether components of the MRN complex influence NHEJ, we used siRNA to deplete Mre11 (siMre11) or Nbs1 (siNbs1) and measured the effect on I-SceI-induced NHEJ. Depletion of Mre11 reduced NHEJ in both Xrcc4+/+ and Xrcc4−/− cells, whether the reporter was sGEJ or vGEJ, in comparison to control luciferase siRNA (siLuc) (Fig. 1b,c). siNbs1 reduced NHEJ only in Xrcc4+/+ cells, but Nbs1 depletion was incomplete; therefore a role for Nbs1 in Xrcc4-independent NHEJ, as observed recently in the context of V(D)J recombination, cannot be excluded (Fig. 1d) 31. Interestingly, the basal level of Mre11 was lower in Xrcc4−/− cells than in wild-type controls, the reasons for which are unclear. Despite this, robust knock-down of Mre11 by siMre11 was observed in each genetic background. Depletion of Mre11 or Nbs1 in HR reporter ES cells produced the expected reduction in I-SceI-induced HR (Supplementary Fig. 3a). The effects of Mre11 or Nbs1 depletion on NHEJ were also observed with use of a second set of independent siRNAs specific for Mre11 and Nbs1 (Supplementary Fig. 3b). Taken together, the data implicates Mre11 in both Xrcc4-dependent and Xrcc4-independent NHEJ, in mouse ES cells.
MRN activates the Atm signaling kinase at DSBs 3, and the effect of MRN deficiency on V(D)J recombination mimicks that of Atm deficiency 32. To determine whether Mre11 regulates NHEJ indirectly through Atm signaling, we examined NHEJ in the presence of the Atm inhibitor, KU55933. Despite evidence of Atm inhibition in KU55933-treated samples, no clear effect of KU55933 on NHEJ was observed in either Xrcc4+/+ or Xrcc4−/− cells (Supplementary Fig. 4). Therefore, the NHEJ function of Mre11 does not require Atm signaling. However, a scaffolding function for Atm, or a qualitative contribution of Atm to NHEJ, as observed in V(D)J recombination 32, has not been excluded by our experiments.
To determine how Mre11 affects NHEJ qualitatively, we used FACS to clone individual I-SceI-induced GFP+ sGEJ reporter cells that had been co-transfected with either siMre11 or control siLuc, then expanded clones for preparation of genomic DNA (gDNA) and breakpoint sequencing. In Xrcc4+/+ sGEJ reporter cells that had received control siLuc, 70% (49/70) of I-SceI-induced NHEJ were precise (Table 1 and Supplementary Fig. 5). Consistent with previous work, only 10.2% (5/49) of I-SceI-induced NHEJ events in isogenic Xrcc4−/− sGEJ reporter cells were precise 14. The remaining imprecise NHEJ events entailed deletions or, in a minority of cases, frame-shifts involving only the second I-SceI site (Table 1, Supplementary Fig. 5 and Supplementary Table 1). In Xrcc4+/+ sGEJ reporter cells that received control siLuc, 76% (16/21) of imprecise NHEJ events entailed MMEJ. In isogenic Xrcc4−/− sGEJ reporter cells, 92% (35/38) of deletional repair events entailed MMEJ and 9% (6/64) of all imprecise rejoining events contained insertions at the breakpoint. Mre11 depletion did not affect the proportions of precise NHEJ in either Xrcc4+/+ or Xrcc4−/− cells; however, Mre11 depletion reduced use of MMEJ in Xrcc4+/+ sGEJ reporter cells, in comparison to control siLuc-treated cells (Table 1 and Supplementary Fig. 5). In siMre11-treated cells, 53% (9/17) of imprecise deletional NHEJ events entailed MMEJ, whereas the equivalent frequencies in control siLuc-treated cells was 76% (16/21; P = 0.027 by X2 test). This effect is reminiscent of the known role of yeast Mre11 in MMEJ 27.
To examine whether Mre11 influences processing of the DSB, we developed a new assay to quantify end processing in bulk cultures of sGEJ reporter cells undergoing I-SceI-induced NHEJ (Fig. 2). Near the tandem I-SceI sites are restriction sites for EcoRI, BglII and PstI. These sites are located to the left of the proximal I-SceI site at distances of 17 bp (EcoRI), 37 bp (BglII) and 64 bp (PstI) (Fig. 2a,b). If resection of the left I-SceI-induced DSB were limited to less than 17 nt, the EcoRI site within the reporter would remain intact. In this case, gDNA restricted with NotI and EcoRI would generate a GFP-hybridizing band of 759 bp by Southern blotting. In contrast, in a clone that had undergone ≥ 17 nt resection of the left end of the DSB, the EcoRI site would be lost and gDNA restricted with NotI and EcoRI would reveal a larger GFP-hybridizing band of ∼3.2 kb by Southern blotting. This provides a way to quantify end processing during I-SceI-induced NHEJ (Fig. 2). We transfected Xrcc4+/+ sGEJ reporter cells and isogenic Xrcc4−/− sGEJ reporter cells with an I-SceI plasmid and either siMre11, control siLuc or siRNA (siCtIP) to deplete the end processing protein, CtIP 21. We FACS sorted pools of I-SceI-induced GFP+ NHEJ products from each test group and prepared gDNA from these pools of cells. We digested gDNA from the different test groups with NotI alone or, in parallel, with NotI + I-SceI, NotI + EcoRI, NotI + BglII, or NotI + PstI, and analyzed restriction digested gDNA by Southern blotting, using a GFP probe (Fig. 2b).
As expected, deletion of Xrcc4 generated more NHEJ products lacking the restriction sites close to the I-SceI site in the sGEJ reporter, as reflected by an increased intensity of the “uncut” ∼3.2 kb band in comparison to Xrcc4+/+ test groups (Fig. 2b). In Xrcc4−/− cells, we noted a reduction in the intensity of this “uncut” band in siMre11-treated groups in comparison to those receiving either control siLuc or siCtIP (Fig. 2b). This suggests that Mre11 depletion reduces the probability that end processing will extend to the restriction sites close to the I-SceI site. We used phosphoimager and densitometry to quantify this effect, by measuring the relative intensity of 32P-hybridizing bands for each treatment group. The probability that end processing had ablated a given restriction site was calculated as the ratio: [“Uncut”:(“Cut” + “Uncut”) × 100]% (Fig. 2b,c and Supplementary Fig. 6). The results suggest that Mre11 promotes end processing during Xrcc4-independent NHEJ. In contrast, depletion of Mre11 in Xrcc4+/+ cells did not diminish end processing in this assay. The reasons for this difference are not yet clear. However, MRN may have both scaffolding and enzymatic functions at the break, each of which might contribute to NHEJ 24,34,35.
Mammalian MRN binds the γ-H2A.X-interacting adaptor protein, Mdc1, during the H2A.X-dependent chromatin response to a DSB 4, and long-range end joining during class switch recombination (CSR) or fusion of dysfunctional telomeres is impaired in H2A.X−/− or Mdc1−/− cells 36-38. To determine whether the fraction of MRN that is associated with γ-H2A.X/Mdc1 contributes to NHEJ, we established an intact, single-copy NHEJ reporter sGEJ in H2A.Xflox/flox mouse ES cells, where H2A.X alleles can be conditionally deleted 39, and used Cre treatment to generate isogenic H2A.X−/− sGEJ reporter clones. Homozygous deletion of H2A.X, whether or not accompanied by expression of wild-type H2A.X or the S139A mutant in H2A.X−/− sGEJ reporter cells, had no quantitative impact on NHEJ (Fig. 3a and Supplementary Fig. 7). Depletion of Mre11, Nbs1 or Brca1 reduced the efficiency of NHEJ in H2A.X+/+ cells as efficiently as in isogenic H2A.X−/− cells (Fig. 3b), and we noted proportionate reductions in I-SceI-induced HR in H2A.X+/+ and isogenic H2A.X−/− HR reporter cells (Fig. 3c). The basal level of Mre11 was slightly reduced in H2A.X−/− cells compared to wild-type cells, the reasons for which are unclear. However, depletion of Mre11 was equally efficient in these two isogenic ES cell lines (Fig. 3d). These results suggest that the key NHEJ and HR functions of Mre11, Nbs1 and Brca1 are executed independently of H2A.X.
Using a single-copy, chromosomally integrated NHEJ reporter, in which NHEJ can be triggered by a site-specific DSB, we show that Mre11 is required for efficient Xrcc4-dependent and Xrcc4-independent NHEJ in mouse ES cells. Southern analysis of NHEJ in Xrcc4−/− cells reveals that Mre11 depletion reduces the extent of deletions associated with error-prone NHEJ. This suggests that endogenous Mre11 normally contributes to processing of the DSB during Xrcc4-independent NHEJ. We find that both the NHEJ and HR functions of Mre11 and Nbs1 are independent of H2AX.
MRN is one of many DSB response protein complexes that accumulate on chromatin marked by γ-H2AX (the “chromatin domain” of the DSB response – Fig. 4). γ-H2AX and its adaptor protein, Mdc1, have defined functions in DSB repair during sister chromatid recombination and CSR 37,40-43. Interestingly, although the association of MRN, 53bp1 and Brca1 with the “chromatin domain” of the DSB response is dependent on H2A.X, these proteins can also accumulate in the “DNA domain” of the DSB response (Fig. 4) in cells lacking H2A.X or Mdc15,44. Importantly, the DSB repair functions of MRN, 53bp1 and Brca1 are in part independent of H2A.X (Fig. 3) 37,42,43, suggesting that MRN, 53bp1 and Brca1 execute their DSB repair functions primarily in association with the “DNA domain” of the DSB response.
The roles of Mre11 in DSB repair have been studied extensively in lower eukaryotes. S. cerevisiae mre11 null mutants reveal inefficient religation of both cohesive and mismatched ends, and this is reversed by expression of either wild-type or nuclease-defective mre11 alleles 25,45,46. This suggests that one function of Mre11 in NHEJ is as a scaffold to support synapsis 46-48. In contrast, mre11 nuclease-defective mutants reveal a defect in MMEJ, suggesting that the Mre11 nuclease resects DNA ends and exposes tracts of microhomology 46,49. In the experiments reported here, Mre11 depletion in Xrcc4+/+ mammalian cells reduced the efficiency of NHEJ but did not affect the strong preference for precise end joining. Since precise NHEJ does not entail end resection, the role of Mre11 in this pathway may be to support synapsis between the two DNA ends, thereby promoting religation of the DSB (Fig. 4). A recent structural analysis of Pyrococcus furiosus Mre11 is consistent with such a role for Mre11 24. In contrast, in Xrcc4−/− cells, Mre11 appears to have additional functions in DSB processing, leading to the generation of extensive deletions during error-prone NHEJ or MMEJ (Fig. 4). Definitive evidence of the relative contributions of Mre11 scaffolding and nuclease functions to mammalian NHEJ will require complementation experiments in cells lacking Mre11.
Mammalian NHEJ factors repair unscheduled chromosomal DSBs and also mediate the fusion of dysfunctional telomeres and specialized recombination reactions in the immune system, such as V(D)J recombination and CSR 11,12,50. It is not yet clear to what extent the “rules” governing one NHEJ process apply to other contexts. Our results illustrate a difference between short-range NHEJ (studied here), which is H2A.X-independent, and long-range NHEJ processes that are partially H2A.X-dependent 36-38. It will be interesting to determine how the distance between the two DSBs influences the quality of DSB repair and its genetic dependencies. Similarly, the regulation of NHEJ has been shown to vary according to cell type 51,52, raising the possibility that our findings are restricted to mouse ES cells. However, recent work implicates MRN in NHEJ in several different cell types, suggesting a general role in NHEJ31,32[+ 53, 54 Ferguson, Lopez papers in press, NSMB]. In addition to the effects on DNA damage signaling and HR, Mre11 dysfunction may promote genomic instability and cancer in mammals by disabling NHEJ.
To construct the NHEJ reporter sGEJ, we purchased from Invitrogen the 70-mer oligo containing two sequential I-SceI sites and the artificial Kozak-ATG translation sequence (5′-cggaattcattaccctgttatccctaaccgccgccaccatggattaccctgttatccctacggatcc-3′) and its complementary 70-mer, annealed, digested with EcoRI and BamHI and inserted into the EcoRI-BamHI site of pcDNA3-EGFP-SnaBI where a silence mutation was introduced into the GFP gene by PCR to generate an SnaBI site (5′-tacgta-3′) using oligo 5′-ccaccctcgtgaccacccttacgtacggc-3′. We replaced the original GFP translation site (5′-gccaccatggtg-3′) with 5′-cttcacatgatc-3′ by PCR using primers 5′-tgggatccatccttcacatgatcagcaagggcgaggagctgttc-3′ and 5′-gccgtacgtaagggtggtcacgagggtgg-3′ to generate pcDNA3-sGEJ. We generated the final ROSA26-PA NHEJ targeting vector sGEJ using an approach described previously 42. We similarly constructed the NHEJ reporter vGEJ containing two inverted I-SceI sites. The hygromycin resistant (HygR) expression vectors for Xrcc4 and HA-tagged wild-type H2A.X and its S139A mutant were constructed as described previously 42,43. We purchased RNAi duplex targeting luciferase control (cgtacgcggaatacttcga), mouse Mre11 (#1: gctgcttggagctgcttag; #2: acaggagaagagatcaact), Nbs1 (#1: gacaggagatagagttacc; #2: gcagttgaatctaagaaac), Brca1 (ccagaagaaagggccttca) and CtIP (ggaactctggacaaaacta) from Dharmacon.
We grew Xrcc4flox/flox and H2A.Xflox/flox mouse ES cells 33,39 in ES medium on either mouse embryonic fibroblast (MEF) feeder cells or gelatinized plates. We established Xrcc4flox/flox and H2A.Xflox/flox NHEJ reporter ES clones harboring a single, intact, randomly integrated copy of the NHEJ reporter as described previously for the establishment of the HR reporter mouse ES cells 42,43. We generated isogenic Xrcc4+/+ and Xrcc4−/− and isogenic H2A.X+/+ and H2A.X−/− ES reporter clones using adeno-Cre infection as described previously 42,43. To generate Xrcc4−/− ES cells stably expressing wild-type human XRCC4, we electroporated 6 × 106 Xrcc4−/− ES cells with 8 mg of HygR XRCC4 expression vector and seeded them on a 10 cm plate. We added hygromycin (400 μg ml−1) 48 hr later to select HygR clones which were expanded ∼7 days later.
Commercial antibodies used in this study were mouse monoclonal anti-β-actin (Cat# ab8226) and rabbit polyclonal anti-Mre11 (Cat# ab397) from Abcam, rabbit polyclonal anti-Nbs1 (Cat# NB100-60648) from Novus Biologicals, rabbit polyclonal anti-HA-tag (Cat# sc-805) and anti-p53 (Cat# sc-6243) and goat polyclonal anti-human XRCC4 (Cat# sc-8285) from Santa Cruz Biotechnology, rabbit polyclonal anti-histone H4 (Cat# 06-598) from Upstate, and rabbit polyclonal anti-phospho-p53 Ser15 (Cat# 9284) from Cell Signaling. Rabbit polyclonal anti-mouse Brca1 antibody was from Junjie Chen. We analyzed histones and non-histone proteins by Western blotting as described before 42.
To sequence repair junctions of I-SceI-induced NHEJ products, we sorted individual I-SceI-induced GFP+ cells using a 4-laser BD FACSAria (BD Biosciences) and expanded them as clones. We prepared gDNA from expanded GFP+ clones as described previously 42. We amplified sequences containing the repair junctions by PCR using primers #1 (tgcacgcttcaaaagcgcacg) and #2 (ctcctggacgtagccttcggg). We purified PCR products and had them sequenced using nested primers #3 (ccgcgctgttctcctcttc) and #4 (gccgtacgtaagggtggtcacgagggtgg). For Southern blotting, we FACS sorted I-SceI-induced GFP+ cells as pools and expanded. We purified gDNA from the sorted, expanded pools of GFP+ cells, digested with relevant restriction enzymes and analyzed by Southern blotting using the GFP cDNA as a probe 42.
To analyze I-SceI-induced GFP+ frequencies in NHEJ or HR reporter mouse ES cells, we transfected I-SceI expression plasmids into these reporter cells as described previously 42,43 to induce a break and thus NHEJ or HR42,43. We performed parallel transfection of a wild-type GFP expression vector, at an amount one tenth of that of the I-SceI expression vector, to determine transfection efficiency. Unless otherwise stated, statistical analysis was by Student's two-tailed unpaired t-test (unknown variance) or, for paired samples, by Student's two-tailed paired t-test.
We prepared Atm kinase inhibitor KU55933 (Calbiochem, Cat# 118500) and DNA-PKcs inhibitor NU7026 (Sigma, Cat# N1537) in DSMO as 10 mM stock solutions and diluted to a final concentration of 20 μM for KU55933 and 40 μM for NU7026 for I-SceI-induced repaired assays. In these assays, we added these inhibitors at 22 hrs post-transfection. After incubation for additional 72 hrs, we analyzed cells for NHEJ levels using flow cytometry. For effect of Atm inhibition on ionizing radiation (IR)-induced phosphorylation of p53 Ser15 and H2A.X Ser139, we preincubated cells in media with chemical inhibitors for 1 hr, exposed to 5 Gy of irradiation, incubated for another 2 hrs and lysed for Western blotting.
We thank Dr. Frederick Alt, Dr. Catherine Yan and members of the Scully lab for helpful discussions, and Drs. Bernard Lopez, David Ferguson and Sandy Chang for discussions and for sharing data prior to publication. This work was supported by R01s CA095175 and GM073894 and a Leukemia and Lymphoma Society Scholar Award (to R.S.).
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Ferguson paper on Mre11, in press, NSMB.
Lopez paper on Mre11, in press, NSMB.
I-SceI-induced NHEJ products in sGEJ reporter cells
|Number of repair events||Percentage of all NHEJ events (%)|
|Total repair events||70||66||49||64||100.0||100.0||100.0||100.0|
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