Document Detail


Role of mammalian Ecdysoneless in cell cycle regulation.
MedLine Citation:
PMID:  19640839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Ecdysoneless (Ecd) protein is required for cell-autonomous roles in development and oogenesis in Drosophila, but the function of its evolutionarily conserved mammalian orthologs is not clear. To study the cellular function of Ecd in mammalian cells, we generated Ecd(lox/lox) mouse embryonic fibroblast cells from Ecd floxed mouse embryos. Cre-mediated deletion of Ecd in Ecd(lox/lox) mouse embryonic fibroblasts led to a proliferative block due to a delay in G(1)-S cell cycle progression; this defect was reversed by the introduction of human Ecd. Loss of Ecd led to marked down-regulation of E2F target gene expression. Furthermore, Ecd directly bound to Rb at the pocket domain and competed with E2F for binding to hypophosphorylated Rb. Our results demonstrate that mammalian Ecd plays a role in cell cycle progression via the Rb-E2F pathway.
Authors:
Jun Hyun Kim; Channabasavaiah Basavaraju Gurumurthy; Mayumi Naramura; Ying Zhang; Andrew T Dudley; Lynn Doglio; Hamid Band; Vimla Band
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-07-29
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  284     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-21     Completed Date:  2009-11-06     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  26402-10     Citation Subset:  IM    
Affiliation:
Department of Genetics, College of Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198-5805, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carrier Proteins / genetics,  metabolism,  physiology*
Cell Cycle / physiology*
Cell Cycle Proteins / genetics,  metabolism
Cell Proliferation*
Cells, Cultured
E2F1 Transcription Factor / genetics,  metabolism
Female
Fibroblasts / cytology,  metabolism*
G1 Phase / physiology
Gene Expression
Humans
Immunoblotting
Immunoprecipitation
Male
Mice
Mice, Knockout
Mice, Transgenic
Phosphorylation
Protein Binding
Retinoblastoma Protein / genetics,  metabolism
S Phase / physiology
Time Factors
Grant Support
ID/Acronym/Agency:
CA105489/CA/NCI NIH HHS; CA116552/CA/NCI NIH HHS; CA87986/CA/NCI NIH HHS; CA94143/CA/NCI NIH HHS; CA99163/CA/NCI NIH HHS; CA99900/CA/NCI NIH HHS; R01 CA105489-08/CA/NCI NIH HHS; R01 CA96844-06/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Cell Cycle Proteins; 0/E2F1 Transcription Factor; 0/ECD protein, human; 0/Retinoblastoma Protein
Comments/Corrections

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