Document Detail

Role of lysophosphatidic acid acyltransferase 3 for the supply of highly polyunsaturated fatty acids in TM4 Sertoli cells.
MedLine Citation:
PMID:  20705908     Owner:  NLM     Status:  MEDLINE    
Sertoli cells supply germ cells with nutrients, including highly polyunsaturated fatty acids (hPUFAs), which are essential for testicular function. We have previously reported high expression of lysophosphatidic acid acyltransferase (LPAAT)3 in mature mouse testis and suggested an arachidonoyl-transferase activity to LPA. To investigate the role of LPAAT3 in the storage and release of PUFAs, TM4 Sertoli cells were stably transfected with LPAAT3-small hairpin (sh)RNA. Arachidonoyl-, eicosapentaenoyl-, and docosapentaenoyl-containing phosphatidylcholine (PC) and linoleoyl-containing phosphatidylethanolamine (PE), phosphatidylserine (PS), and phosphatidylglycerol were significantly decreased as determined by liquid chromatography coupled to electrospray ionization mass spectrometry. Expression of murine LPAAT3 in Chinese hamster ovary (CHO)-K1 cells had essentially an opposite effect. The level of polyunsaturated PC correlated with cellular levels of free docosapentaenoic acid and eicosapentaenoic acid in TM4 and CHO-K1 cells, respectively. Activity assays using microsomal preparations as a source of LPAAT3 revealed an excessive PA synthesis from LPA acceptors for docosahexaenoyl-, arachidonoyl- and less pronounced for linoleoyl-CoA. We propose that the efficient incorporation of hPUFAs into PA-the precursor of several phospholipids, including PC-and the selective increase of the polyunsaturated PC pool in TM4 Sertoli cells might be required for the controlled release of hPUFAs and their supply to germ cells.
Andreas Koeberle; Hideo Shindou; Takeshi Harayama; Takao Shimizu
Related Documents :
15262288 - Metabolic pathways for dietary lipids in the midgut of hematophagous panstrongylus megi...
3924098 - The effect of acidic lipids on the activity of bovine milk galactosyltransferase in ves...
565218 - The synthesis of phosphatidylcholine by adult rat lung alveolar type ii epithelial cell...
7943658 - Agonist-stimulated and basal phosphatidylethanol formation in neutrophils from alcoholics.
6418148 - Conversion of arachidonic acid to lipoxygenase products by human fetal tissues.
9404078 - Antioxidant activity of ferulic acid beta-glucuronide in the ldl oxidation system.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-12
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  24     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-02     Completed Date:  2011-01-04     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4929-38     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Tokyo, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acyltransferases / genetics,  metabolism*
Blotting, Western
CHO Cells
Cell Line
Cell Proliferation
Chromatography, Liquid
Fatty Acids, Unsaturated / metabolism*
Fluorescent Antibody Technique
Microsomes / metabolism
Polymerase Chain Reaction
Sertoli Cells / cytology,  enzymology*
Tandem Mass Spectrometry
Reg. No./Substance:
0/Fatty Acids, Unsaturated; EC 2.3.-/Acyltransferases; EC acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Random tree-puzzle leads to the yule-harding distribution.
Next Document:  A Comparison of Global, Gene-Specific, and Relaxed Clock Methods in a Comparative Genomics Framework...