Document Detail

Role of lymphocytes in the course of murine zymosan-induced peritonitis.
MedLine Citation:
PMID:  18516709     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE AND DESIGN: To investigate a putative role of lymphocytes in a murine model of zymosan peritonitis. MATERIAL OR SUBJECTS: Rag-deficient mice (KO) and their counterparts (WT) (13 animals in each group). TREATMENT: Mice were injected i. p. with zymosan (2 mg/ml, 0.5 ml/mouse) and sacrificed either 30 min or 6 h post-treatment. METHODS: At 30 min of inflammation vascular permeability was assessed by peritoneal leakage of i. v. injected Evans blue. At 6 h of peritonitis leukocyte numbers were estimated (Turk's staining), and MMP-2 and -9 presence (zymography). Levels of inflammatory mediators were evaluated by either ELISA (PGE(2), KC) or Cytometric Bead Array (IL-6, IL-10, MCP-1, IFN-gamma, TNF-alpha, and IL-12p70). The Amount of nitric oxide (NO) was measured by the Greiss reaction. Differences between WT and KO mice were analyzed by Student's t-test (p </=0.05). RESULTS: During zymosan peritonitis, there was a decreased production of IFN-alpha (p = 0.03) and IL-10 (p = 0.03) and elevated synthesis of NO (p = 0.0001) in KO mice compared with WT controls. Despite this, no alterations in major events of peritonitis (vascular permeability and neutrophil infiltration) were detected in KO mice. CONCLUSIONS: Lymphocytes do not have a significant impact on zymosan peritonitis in mice.
E Kolaczkowska; M Barteczko; B Plytycz; B Arnold
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Inflammation research : official journal of the European Histamine Research Society ... [et al.]     Volume:  57     ISSN:  1023-3830     ISO Abbreviation:  Inflamm. Res.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-07-02     Completed Date:  2008-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508160     Medline TA:  Inflamm Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  272-8     Citation Subset:  IM    
Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, ul. Ingardena 6, PL-30-060 Krakow, Poland.
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MeSH Terms
Capillary Permeability
Chemokines / metabolism
Cytokines / metabolism
Dinoprostone / metabolism
Homeodomain Proteins / genetics,  metabolism
Lymphocytes / immunology*
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 9 / metabolism
Mice, Inbred C57BL
Mice, Knockout
Nitric Oxide / metabolism
Peritonitis* / chemically induced,  immunology
Protein Array Analysis
Zymosan / pharmacology*
Reg. No./Substance:
0/Chemokines; 0/Cytokines; 0/Homeodomain Proteins; 10102-43-9/Nitric Oxide; 128559-51-3/RAG-1 protein; 363-24-6/Dinoprostone; 9010-72-4/Zymosan; EC Metalloproteinase 2; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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