Document Detail

Role of liver and plasma lipoproteins in selective transport of n-3 fatty acids to tissues: a comparative study of 14C-DHA and 3H-oleic acid tracers.
MedLine Citation:
PMID:  17901547     Owner:  NLM     Status:  MEDLINE    
We conducted a study aimed at a direct comparison of the plasma dynamics and uptake of docosahexaenoic (DHA) and oleic (OA) fatty acids by various organs. 14C-DHA and 3H-OA were intravenously co-injected into mice. At 5 min after injection, more than 40% of the 14C-DHA, but less than 20% of the 3H-OA, labels was associated with the liver. Heart uptake of 14C-DHA was three to four times greater compared to the 3H-OA label. Brain incorporation of 14C-DHA slowly rose to 0.7% at 24 h, but it remained at the 1-1.5% level for 3H-OA. Total 14C activity in plasma reached 2% of the injected dose at 20 min and leveled off at 0.5% after 1.5 h. Fifteen percent of 14C-DHA plasma activity at 30 min was associated with non-esterified fatty acids, whereas about 85% was recovered in triglycerides in very low-density lipoprotein (VLDL) and LDL fractions. Only 30% of 3H-OA derived activity was found in the VLDL fraction at 30 min. All 3H activity in plasma at later time points was in catabolite fractions. These findings demonstrate that liver plays an important role in the initial selectivity for DHA. It is likely that DHA is specifically taken up by liver, esterified, loaded into lipoproteins, and then delivered to brain, heart, and other target tissues.
Alla Polozova; Norman Salem
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of molecular neuroscience : MN     Volume:  33     ISSN:  0895-8696     ISO Abbreviation:  J. Mol. Neurosci.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-28     Completed Date:  2008-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002991     Medline TA:  J Mol Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  56-66     Citation Subset:  IM    
Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, 5625 Fishers Lane, Bethesda, MD 20892-9410, USA.
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MeSH Terms
Biological Transport / physiology
Brain / metabolism
Carbon Radioisotopes / chemistry,  metabolism
Docosahexaenoic Acids / chemistry,  metabolism*
Fatty Acids, Omega-3 / metabolism*
Lipoproteins / chemistry,  metabolism*
Liver / chemistry,  metabolism*
Mice, Inbred C57BL
Myocardium / chemistry,  metabolism
Oleic Acid / chemistry,  metabolism*
Plasma / chemistry,  metabolism*
Tissue Distribution
Tritium / chemistry,  metabolism
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fatty Acids, Omega-3; 0/Lipoproteins; 10028-17-8/Tritium; 112-80-1/Oleic Acid; 25167-62-8/Docosahexaenoic Acids

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