Document Detail

Role of lipocortin I in the glucocorticoid induction of the terminal differentiation of a human squamous carcinoma.
MedLine Citation:
PMID:  2137129     Owner:  NLM     Status:  MEDLINE    
The human squamous cell carcinoma SqCC/Y1 undergoes spontaneous terminal differentiation in the confluent state. The degree of maturation was markedly increased by glucocorticoids and by both human recombinant and placental lipocortin I. Western analyses demonstrated cellular secretion of lipocortin into the medium. Glucocorticoid-induced maturation was antagonized by a lipocortin I-specific monoclonal antibody, by phospholipase A2 (PLA2), and by arachidonic acid. Induction of the differentiation of SqCC/Y1 cells by lipocortin I was prevented by arachidonic acid. The PLA2 inhibitor, dibromoacetophenone, caused an increase in envelope-competent cells indicating that inhibition of PLA2 results in induction of differentiation. Epidermal growth factor prevented the induction of differentiation by both lipocortin I and by glucocorticoids. The nonsteroidal lipoxygenase/cyclo-oxygenase inhibitor, phenidone, also increased SqCC/Y1 differentiation, suggesting that leukotrienes, thromboxanes, and/or prostaglandins may be involved in lipocortin-mediated regulation of SqCC/Y1 maturation. The findings support a role for lipocortin I in mediating the effects of glucocorticoids on epidermal cell differentiation.
S M Violette; I King; J L Browning; R B Pepinsky; B P Wallner; A C Sartorelli
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  142     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-02-28     Completed Date:  1990-02-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  70-7     Citation Subset:  IM    
Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06510.
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MeSH Terms
Antibodies, Monoclonal / immunology
Arachidonic Acid
Arachidonic Acids / pharmacology
Calcium-Binding Proteins / immunology,  physiology*
Carcinoma, Squamous Cell / pathology*,  physiopathology
Cell Line
Cell Transformation, Neoplastic / drug effects*
Glucocorticoids / pharmacology*
Phospholipases / antagonists & inhibitors*
Phospholipases A / pharmacology
Phospholipases A2
Tumor Cells, Cultured / pathology
Grant Support
Reg. No./Substance:
0/Annexins; 0/Antibodies, Monoclonal; 0/Arachidonic Acids; 0/Calcium-Binding Proteins; 0/Glucocorticoids; 506-32-1/Arachidonic Acid; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC A2

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