| Role of leukotriene B4 in celecoxib-mediated anticancer effect. | |
MedLine Citation:
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PMID: 20937254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, has anticancer effect on many cancers associated with chronic inflammation by both COX-2-dependent and COX-2-independent mechanisms. The non-COX-2 targets of celecoxib, however, are still a matter of research. Leukotriene B4 (LTB4) has been implicated in prostate and colon carcinogenesis, but little is known about the potential role of LTB4 in celecoxib-mediated anticancer effect. In this study, we evaluated whether LTB4 was involved in celecoxib-mediated inhibitory effect on human colon cancer HT-29 cells and human prostate cancer PC-3 cells. Our data showed that survival of both cell lines was obviously suppressed after celecoxib treatment for 72 h in a concentration-dependent manner. However, only in HT-29 cells, this inhibitory effect could be reversed by LTB4, which promoted survival of HT-29 cells rather than PC-3 cells. Consistent with these results, lioxygenase (LOX) potent inhibitor nordihydroguaiaretic acid (NDGA) had a higher inhibitory effect on HT-29 cells than PC-3 cells. Additionally, ELISA results showed that celecoxib could suppress expression of LTB4 in both cell lines, whereas, inhibition of PGE2 was only detected in HT-29 cells. These results indicate that the anticancer effect of celecoxib is COX-2-independent in HT-29 and PC-3 cells and in HT-29 cells primarily via down-regulating LTB4 production. |
Authors:
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Peng Gao; Lei Guan; Jie Zheng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-19 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 402 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-15 Completed Date: 2010-12-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 308-11 Citation Subset: IM |
Copyright Information:
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Crown Copyright © 2010. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Pathology and Pathophysiology, School of Medical Science, Southeast University, Nanjing 210009, Jiangsu, People's Republic of China. gp_yaya@163.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Cell Line, Tumor Cell Proliferation / drug effects* Cell Survival / drug effects Colonic Neoplasms / metabolism Cyclooxygenase 2 Inhibitors / pharmacology* Humans Leukotriene B4 / antagonists & inhibitors, metabolism* Lipoxygenase Inhibitors / pharmacology Male Nordihydroguaiaretic Acid / pharmacology Prostatic Neoplasms / metabolism Pyrazoles / pharmacology* Sulfonamides / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Cyclooxygenase 2 Inhibitors; 0/Lipoxygenase Inhibitors; 0/Pyrazoles; 0/Sulfonamides; 169590-42-5/celecoxib; 500-38-9/Nordihydroguaiaretic Acid; 71160-24-2/Leukotriene B4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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