Document Detail

Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion.
MedLine Citation:
PMID:  11179066     Owner:  NLM     Status:  MEDLINE    
We investigated leukocyte involvement in uterine hypoperfusion and intrauterine fetal growth retardation (IUGR) induced by ischemia-reperfusion (I/R) in Sprague-Dawley rats. On day 17 of gestation, leukocyte accumulation in the uterus and placenta subjected to 30 min of ischemia, followed by reperfusion, was assessed by measuring myeloperoxidase (MPO) activity. Uterine MPO activity was significantly higher after 1 h of reperfusion than it was before ischemia (P < 0.05), without any increase in placental MPO activity. Immunohistochemical staining showed leukocyte accumulation in the uterus subjected to I/R. The effects of treatment with monoclonal antibodies against CD11a (WT1) and CD18 (WT3) at a dose of 0.8 mg/kg on uterine blood flow and IUGR were investigated. Laser-Doppler flowmetry demonstrated that uterine hypoperfusion at 2 h after ischemia (blood flow, -51.7 +/- 1.2%; P < 0.01) was inhibited by WT1 and WT3 treatment. I/R-induced IUGR at full term (P < 0.05 vs. nonischemic horn) was prevented by WT1 and WT3 treatment on day 17. These results indicate that leukocyte accumulation may play an important role in the pathogenesis of uterine hypoperfusion and IUGR induced by I/R in pregnant rats.
K Miyakoshi; H Ishimoto; O Nishimura; S Tanigaki; M Tanaka; T Miyazaki; M Natori; Y Yoshimura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  280     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1215-21     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan.
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MeSH Terms
Antibodies, Monoclonal / pharmacology
Antigens, CD18 / immunology
Fetal Growth Retardation / immunology*,  pathology,  physiopathology*
Fetal Weight
Leukocytes / physiology*
Lymphocyte Function-Associated Antigen-1 / immunology
Organ Size
Placenta / blood supply,  immunology,  pathology
Reperfusion Injury / immunology*,  pathology,  physiopathology*
Uterus / blood supply*,  immunology,  physiopathology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD18; 0/Lymphocyte Function-Associated Antigen-1

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