Document Detail


Role of katp channels and TRPV1 receptors in hydrogen sulfide-enhanced gastric emptying of liquid in awake mice.
MedLine Citation:
PMID:  22884438     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Hydrogen sulphide (H(2)S) has shown to relax gastrointestinal muscle. Here in, we evaluated the effects of H(2)S donors on gastric emptying and in pyloric sphincter muscle relaxation, and whether these effects involved K(ATP) channels or TRPV1 receptors. Mice were treated with l-cysteine (alone or with propargylglycine-an inhibitor of H(2)S synthesis), NaHS, Lawesson's reagent (H(2)S donors) or saline. After 30min, mice were gavaged with a liquid meal containing a nonabsorbable marker and then killed at 10, 20 or 30min intervals to assess marker recovery from the stomach and intestine. This experiment was repeated in mice pre-treated with K(ATP) channel (glibenclamide) or TRPV1 receptor (capsazepine) antagonists. In addition, pyloric sphincter muscles were mounted in an organ bath, incubated with saline, glibenclamide or capsazepine, and NaHS dose-responses were determined. H(2)S donors and l-cysteine enhanced gastric emptying in a dose-dependent manner; propargylglycine reversed the effect of l-cysteine. Both glibenclamide and capsazepine abolished l-cysteine and H(2)S donors' augmentation of gastric emptying. Dose-dependent inductions of pyloric sphincter relaxation by NaHS were abolished by glibenclamide or capsazepine. These data suggest that H(2)S donors-induced acceleration of gastric emptying and relaxation of pyloric sphincter muscle by K(ATP) channel and TRPV1 receptor activations.
Authors:
Jand Venes R Medeiros; Víctor H Bezerra; Larisse T Lucetti; Roberto César P Lima; André Luiz R Barbosa; Bruno M Tavares; Pedro Jorge C Magalhães; Armênio A Santos; Fernando Q Cunha; Pedro Marcos G Soares; Marcellus Hlp Souza
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-2
Journal Detail:
Title:  European journal of pharmacology     Volume:  -     ISSN:  1879-0712     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Institute of Biomedicine of Brazilian Semi-Arid (INCT-IBISAB), Departament of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, CEP: 60430-270, Fortaleza, CE, Brazil; Biotechnology and Biodiversity Center Research (BIOTEC), Faculty of Biology, Federal University of Piauí, Campus of Parnaíba, Av. São Sebastião, 2819, CEP: 64202-020, Parnaíba, PI, Brazil.
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