Document Detail


Role of intracellular interleukin-1 receptor antagonist in skin carcinogenesis.
MedLine Citation:
PMID:  11346884     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interleukin 1 (IL-1) is a major mediator of inflammation and exerts pleiotropic effects on many systems. To elucidate the role of its endogenous inhibitor, intracellular IL-1 receptor antagonist (icIL-1Ra), in mouse skin, we produced an icIL-1Ra-overexpressing skin carcinoma cell line (icIL-1Ra-JWF2). Altered expression of icIL-1Ra did not change IL-1alpha mRNA levels in these transfected cells. In icIL-1Ra-JWF2 cells, however, cyclooxygenase-2 mRNA levels were dramatically reduced and shown to be transcriptionally regulated by icIL-1Ra. To determine the effect of icIL-1Ra on cell proliferation, cell counts were done 24 h after plating equal numbers of cells. Cells from three icIL-1Ra-JWF2 clones showed significantly reduced growth rates compared with parental JWF2 cells. We subcutaneously injected five independent clones of icIL-1Ra-JWF2 cells into nude mice and measured the tumor doubling time by weekly measurements of tumor volume. IcIL-1Ra appeared to significantly slow the growth of tumors in vivo. Collectively these observations suggest that IL-1Ra has antiproliferative effects in murine skin carcinoma cells.
Authors:
E La; J E Rundhaug; S M Fischer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular carcinogenesis     Volume:  30     ISSN:  0899-1987     ISO Abbreviation:  Mol. Carcinog.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-05-10     Completed Date:  2001-05-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8811105     Medline TA:  Mol Carcinog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  218-23     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Affiliation:
Department of Carcinogenesis, The University of Texas M. D. Anderson Cancer Center Science Park-Research Division, Smithville 78957, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Carcinogenicity Tests / methods*
Carcinoma, Squamous Cell / metabolism*
Cell Division
Cyclooxygenase 2
Female
Interleukin 1 Receptor Antagonist Protein
Isoenzymes / genetics,  metabolism
Keratinocytes / metabolism*
Mice
Mice, Nude
Neoplasm Transplantation
Prostaglandin-Endoperoxide Synthases / genetics,  metabolism
Sialoglycoproteins / genetics,  metabolism*
Skin Neoplasms / metabolism*
Transfection
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA 16672/CA/NCI NIH HHS; CA 60996/CA/NCI NIH HHS; ES 07784/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Il1rn protein, mouse; 0/Interleukin 1 Receptor Antagonist Protein; 0/Isoenzymes; 0/Sialoglycoproteins; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

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