| Role of interleukin-5 in enhanced migration of eosinophils from airways of immunized guinea-pigs. | |
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MedLine Citation:
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PMID: 7858864 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Platelet activating factor (PAF), leukotriene B4 (LTB4) and interleukin-5 (IL-5) are potent chemoattractants for guinea-pig eosinophils, which may be involved in eosinophil recruitment and up-regulation in allergic diseases. Eosinophils from the bronchoalveolar lavage fluid (BALF) of ovalbumin-sensitized guinea-pigs were collected 24 h after antigen provocation and migration induced by PAF, LTB4 and rhIL-5 was studied. 2. Total BALF content and distribution of eosinophils were greater in immunized, ovalbumin-challenged guinea-pigs (5.0 +/- 0.8 x 10(6)/guinea-pig; 12 +/- 1%) than in immunized, saline-challenged animals (3.0 +/- 0.7 x 10(6)/guinea-pig; 7 +/- 1%). 3. The chemoattraction of eosinophils isolated on a metrizamide gradient was studied in micro-Boyden chambers, results being expressed as the number of migrating cells (mean +/- s.e. mean). PAF and LTB4-induced migration of eosinophils from immunized and OA-challenged guinea-pigs were significantly enhanced, as compared to immunized and saline-challenged animals (170 +/- 36 vs 35 +/- 9 migrating eosinophils for 10 nM PAF; 271 +/- 60 vs 110 +/- 19 for 1 nM LTB4). 4. The IL-5 antibody TRFK-5, in vivo, reduced eosinophil recruitment in BALF of antigen-challenged immunized animals as well as the enhanced responsiveness of eosinophils from the challenged animals, suggesting a role for IL-5 in the priming of eosinophils in vivo. 5. In contrast to TRFK-5, nedocromil sodium reduced to a similar extent eosinophil, macrophage and lymphocyte recruitment into the BALF of antigen-challenged, but failed to down-regulate the enhanced responsiveness of eosinophils from the challenged animals. 6. The increased eosinophil content in lungs from antigen-challenged guinea-pigs is thus selectively reduced by the anti-IL-5 antibody, which also attenuates the concomitant enhancement of the eosinophil responsiveness, supporting the concept that IL-5 is essential for recruitment and priming of eosinophils in vivo. In contrast, nedocromil sodium reduced non-selectively the total cell recruitment to the airways,but failed to attenuate the enhanced responsiveness of those eosinophils which migrated, indicating that its effects involve a different target. |
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Authors:
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E Coëffier; D Joseph; B B Vargaftig |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: British journal of pharmacology Volume: 113 ISSN: 0007-1188 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1995-03-21 Completed Date: 1995-03-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 749-56 Citation Subset: IM |
Affiliation:
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Unité de Pharmacologie Cellulaire, Unité Associée Institut Pasteur-INSERM no. 285, Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology Antigens / immunology* Bronchoalveolar Lavage Fluid / cytology* Cell Movement Eosinophils / physiology* Guinea Pigs Immunization Interleukin-5 / physiology* Leukotriene B4 / pharmacology Male Nedocromil / pharmacology Ovalbumin / immunology Platelet Activating Factor / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens; 0/Interleukin-5; 0/Platelet Activating Factor; 69049-73-6/Nedocromil; 71160-24-2/Leukotriene B4; 9006-59-1/Ovalbumin |
| Comments/Corrections | |
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