Document Detail

Role of interleukin-1 in induction of matrix metalloproteinases synthesized by rat temporomandibular joint chondrocytes and disc cells.
MedLine Citation:
PMID:  11330935     Owner:  NLM     Status:  MEDLINE    
To identify cartilage-degrading enzymes and cell types that can be specifically induced by interleukin-1 (IL-1)alpha, we studied matrix metalloproteinase (MMP) activities of cultured rat temporomandibular joint (TMJ) chondrocytes and disc cells. The cells were isolated from TMJs pre-injected with normal physiological saline (CR) or recombinant human IL-1alpha (AR). MMP activities in the conditioned media were assayed by gelatin enzymography, and they were identified by Western blot analyses. MMP mRNAs in these cells were also detected by RT-PCR. IL-1alpha significantly induced an increase of active MMP9 as well as pro- and active MMP3, but had no effect on the MMP2 activity in both types of cells. MMP3 and MMP9 mRNAs were also inducible in these cells by IL-1alpha stimulation. Furthermore, disc cells were more susceptible to IL-1alpha than chondrocytes. AR cells spontaneously produced the same MMPs in vitro as the CR cells synthesized under IL-1alpha stimulation. The results indicate that MMP9 and MMP3 were predominantly produced by disc cells, and these may be considered to play a pivotal role in ECM degradation during pathological conditions of the TMJ, such as IL-1-induced TMJ arthritis.
Y Ijima; M Kobayashi; E Kubota
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of oral sciences     Volume:  109     ISSN:  0909-8836     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-05-01     Completed Date:  2001-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  50-9     Citation Subset:  D; IM    
Second Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, Yokosuka, Japan.
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MeSH Terms
Analysis of Variance
Blotting, Western
Cells, Cultured
Chondrocytes / enzymology
Electrophoresis, Polyacrylamide Gel
Enzyme Induction / drug effects*
Interleukin-1 / pharmacology*,  physiology
Matrix Metalloproteinase 2 / biosynthesis
Matrix Metalloproteinase 3 / biosynthesis
Matrix Metalloproteinase 9 / biosynthesis
Matrix Metalloproteinases / biosynthesis*
RNA, Messenger / analysis
Rats, Wistar
Recombinant Proteins / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Statistics, Nonparametric
Temporomandibular Joint / cytology,  enzymology*
Temporomandibular Joint Disk / cytology,  enzymology
Reg. No./Substance:
0/Interleukin-1; 0/RNA, Messenger; 0/Recombinant Proteins; EC 3.4.24.-/Matrix Metalloproteinases; EC Metalloproteinase 3; EC Metalloproteinase 2; EC Metalloproteinase 9

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