Document Detail


Role of Kenae/CCDC125 in cell motility through the deregulation of RhoGTPase.
MedLine Citation:
PMID:  19787194     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isaac's syndrome is a movement disorder characterized by hyperexcitability of peripheral motor nerves. Patients with Isaac's syndrome often develop auto-antibodies to voltage-gated potassium channels (VGKCs) which block their function. However, anti-VGKC antibodies are not detected in all patients with Isaac's syndrome, suggesting the existence of another etiology. In this study, we performed immunoscreening using the serum from a patient with Isaac's syndrome and identified the novel gene named Kenae/CCDC125. Expression analysis of Kenae/CCDC125 revealed that its transcript was highly expressed in tissues associated with the immune system, such as the thymus, spleen and bone marrow. In cells stably expressing Kenae/CCDC125, delay in cell motility and deregulation of RhoGTPase (RhoA, Rac1 and cdc42) activity to extracellular stimuli were demonstrated. These results suggest that the novel gene, Kenae/CCDC125, acts as a regulator of cell motility through RhoA, Rac1 and cdc42.
Authors:
Natsumi Araya; Hitoshi Arimura; Ko-ichi Kawahara; Naoko Yagishita; Junji Ishida; Ryoji Fujii; Satoko Aratani; Hidetoshi Fujita; Tomoo Sato; Yoshihisa Yamano; Itsuro Higuchi; Mitsuhiro Osame; Kusuki Nishioka; Akiyoshi Fukamizu; Kimiyoshi Arimura; Ikuro Maruyama; Toshihiro Nakajima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  24     ISSN:  1791-244X     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-09-29     Completed Date:  2009-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  605-11     Citation Subset:  IM    
Affiliation:
Department of Genome Science, Institute of Medical Science, St. Marianna University School of Medicine, Kanagawa 216-8512, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB024691
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antigens / blood
Base Sequence
Cell Line, Tumor
Cell Movement*
Cloning, Molecular
GTPase-Activating Proteins / metabolism*
Gene Expression Profiling
Gene Expression Regulation
Humans
Isaacs Syndrome / blood,  metabolism
Mice
Molecular Sequence Data
Proteins / chemistry,  genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Antigens; 0/CCDC125 protein, human; 0/CCDC125 protein, mouse; 0/GTPase-Activating Proteins; 0/Proteins; 0/RNA, Messenger

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