Document Detail


Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
MedLine Citation:
PMID:  11229403     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclear-mitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-beta. We show that importin-beta is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-beta and NuMA. Importin-beta therefore links NuMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis.
Authors:
C Wiese; A Wilde; M S Moore; S A Adam; A Merdes; Y Zheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  291     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-03-01     Completed Date:  2001-03-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  653-6     Citation Subset:  IM    
Affiliation:
Department of Embryology, Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, MD 21210, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Extracts
Cell Nucleus / metabolism
Guanosine Triphosphate / metabolism
Interphase
Karyopherins
Microtubules / drug effects,  metabolism*
Mitosis
Mitotic Spindle Apparatus / drug effects,  metabolism*
Models, Biological
Nuclear Proteins / metabolism*,  pharmacology
Ovum
Paclitaxel / pharmacology
Recombinant Fusion Proteins / metabolism
Xenopus
Xenopus Proteins*
ran GTP-Binding Protein / metabolism*
Grant Support
ID/Acronym/Agency:
GM47866/GM/NIGMS NIH HHS; GM53678/GM/NIGMS NIH HHS; GM56312/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Extracts; 0/Karyopherins; 0/NuMa protein, Xenopus; 0/Nuclear Proteins; 0/Recombinant Fusion Proteins; 0/Xenopus Proteins; 33069-62-4/Paclitaxel; 86-01-1/Guanosine Triphosphate; EC 3.6.5.2/ran GTP-Binding Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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